... Cosmids PACs BACs Bacteriophage Vectors Lambda: Each clone can accept inserts of ~15-20 kb. Replace part of l DNA that is not needed for replication with ...
Date added: January 4, 2015 - Views: 1
... screening labelling PowerPoint Presentation PowerPoint Presentation phage and cosmid screening phages cosmids PACs BACs PowerPoint Presentation YACs ...
Date added: October 12, 2011 - Views: 19
... & Cosmids (plasmid + l cos site & can be pckged into phage capsid) Transgenic Plant: Vector = Agrobacterium tumefaciens plasmid called Ti ...
Date added: August 2, 2013 - Views: 15
Vectors for large DNA inserts: Examples: Cosmids PACs BACs Plasmid Vectors Origin of Replication b-lactamase: breaks down ampicillin ...
Date added: May 13, 2013 - Views: 27
... Life cycle of a bacteriophage 2-8 Constructing a genomic library in phage 2-9 Constructing a genomic library in cosmids 2-10 Constructing a genomic ...
Date added: August 8, 2014 - Views: 3
Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...
Date added: December 2, 2011 - Views: 95
Biotechnology and Genetic Engineering PBIO 450/550 Gene libraries cDNA libraries Library screening Eukaryotic gene organization enhancers silencers Genomic library ...
Date added: October 6, 2011 - Views: 28
... cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation ...
Date added: January 13, 2013 - Views: 22
Title: Biotechnology and Genetic Engineering-PBIO 450/550 Author: showalte Last modified by: Allan Showalter Created Date: 9/8/2003 3:48:03 PM Document presentation ...
Date added: February 13, 2013 - Views: 46
Molecular Basis for Relationship between Genotype and Phenotype DNA genotype DNA sequence transcription RNA translation amino acid sequence protein function
Date added: September 8, 2014 - Views: 2
... (DUP25) region showing STSs and genes surrounding the BACs and cosmids used as FISH probes (Gratacòs et al. 2001). The centromeric (CEN) and telomeric ...
Date added: August 22, 2015 - Views: 1
Genome Sequencing Bacteriophage fX174, the first genome to be sequenced, is a viral genome with only 5,368 base pairs ... a set of cDNA sequences to a set of cosmids, ...
Date added: September 12, 2011 - Views: 67
... replicatioin from two different organisms Can be replicated in both Often used to shuttle plasmids from bacteria to Cosmids – related to bacteriophages ...
Date added: May 6, 2013 - Views: 8
... phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, cDNA Nucleic acid probes - cloned DNA or synthetic oligos “Instant” cloning ...
Date added: November 28, 2013 - Views: 4
Recombinant DNA Technology Needles in Haystacks How to find one gene in large genome? A gene might be 1/1,000,000 of the genome. Three basic approaches: 1.
Date added: October 7, 2011 - Views: 265
... Progress to date: 105 of 319 cosmids fully processed 11493 independent insertions 10459 insertions in 2520 orfs (of 7825 in total) 4.2 ...
Date added: May 23, 2014 - Views: 7
Cosmids combination of lambda phage and plasmid hold inserts 35-45 kb FIGURE 20.6 3. Artificial Chromosomes YAC (yeast artificial Chromosomes - 500 ...
Date added: March 14, 2012 - Views: 78
... but not in the middle Cosmids A cosmid is a plasmid with the l phage cos site included in it The cos site signals for packaging into l phage, ...
Date added: December 10, 2013 - Views: 3
Lambda RED Recombination JIC in Norwich University of Tübingen
Date added: May 15, 2013 - Views: 2
3 Basic Principles of Recombinant DNA Technology. Brief Chapter Outline I. Cause for Concern? Recombinant DNA Technology: Promise and Controversy
Date added: May 26, 2012 - Views: 173
Title: Basic Molecular Biology Information Author: t80maj1 Last modified by: t80maj1 Created Date: 8/21/2006 4:36:06 PM Document presentation format
Date added: October 29, 2011 - Views: 78
From http://www.stat.unc.edu/postscript/papers/marron/Stat321FDA/RimaIzempresentation.ppt Nearest Neighbor ... from chromosome 19p13.2 cosmids R31240, R30272 ...
Date added: November 14, 2011 - Views: 112
Chapter 10: Chromosomes and chromosome errors Making chromosome preparations Normal karyotypes from domestic animals Chromosome errors Identification of chromosomes ...
Date added: May 3, 2013 - Views: 2
Viral Vectors May 8 Viral vectors ... cell-cell spread suppression of class I MHC promoter pA pack HSV-ori bacterial plasmid seq cloning site defective virus cosmids ...
Date added: February 19, 2012 - Views: 42
Title: PowerPoint Presentation Author: Wenun Last modified by: Wenun Created Date: 8/26/2004 6:56:18 AM Document presentation format: On-screen Show
Date added: May 31, 2013 - Views: 2
Whole Genome Shotgun Sequencing cut many times at random genome forward-reverse linked reads plasmids (2 – 10 Kbp) cosmids (40 Kbp) ...
Date added: August 3, 2013 - Views: 6
Cloning in Cosmids. Production of overlapping restriction fragments by partial digestion of human genomic DNA with Sau3A. CHROMOSOME WALKING. CHROMOSOME WALKING.
Date added: June 27, 2015 - Views: 1
Michael Kovshilovsky Swapna Annavarapu SoCalBSI 2005 Internship site: BioDiscovery, Inc. Mentor: Dr. Bruce Hoff Source of Funding: BioDiscovery, Inc. Motivation ...
Date added: August 24, 2014 - Views: 28
Overview Part A rDNA Objectives Steps in rDNA Processing Genetic Engineering Advances Part B The Use of rDNA to Produce Human Insulin ... Cosmids combine essential ...
Date added: September 11, 2011 - Views: 270
A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening bacteria by replica plating Cosmids Other Vectors BACs ...
Date added: January 31, 2015 - Views: 7
cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be
Date added: October 7, 2011 - Views: 229
2.2 - Microarray Data Modeling Lab Sébastien Lemieux Elitra Canada Ltd. Objectives to learn the basic principles about microarray experiments. to understand the main ...
Date added: August 24, 2014 - Views: 34
Питања за испит Типови особина које се уводе у биљке генетским инжењерством Основне класе ...
Date added: February 4, 2015 - Views: 1
Cosmids BACs PACs YACs Cosmids replicate as high copy number plasmids--tend to be unstable, ... cDNA library construction using reverse transcriptase ESTs: ...
Date added: October 7, 2011 - Views: 77
... Linear DNA Can insert up to 15 kb fragments Phage Insertion Cosmids Don’t occur naturally ... Eco R1 Vectors A self-replicating DNA molecule that is ...
Date added: May 3, 2013 - Views: 35
Fosmids offer higher stability than comparable high copy number cosmids. Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.
Date added: October 2, 2014 - Views: 4
Insert size range < 15kb. Bacteriophage-based Cosmids: Linear DNA molecules used for genomic and cDNA cloning. Bacterial host. Insert size range < 20kb. ...
Date added: October 7, 2011 - Views: 115
Chapter 4 Molecular Cloning Methods ... Cosmids can accept even larger inserts—up to 50 kb—making them a favorite choice for genomic libraries.
Date added: January 28, 2012 - Views: 179
Chapter 8 Recombinant DNA and Genetic Engineering Genetic manipulation 7 and 9 February, 2005
Date added: May 15, 2013 - Views: 8
BACTERIOPHAGE LAMBDA COSMID VECTOR Purpose: 1. Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.
Date added: January 23, 2013 - Views: 35
Setting up a transformation--how will the competent cells be treated? No plasmid (negative control, nothing should grow on this plate) Supercoiled plasmid of a known ...
Date added: November 15, 2011 - Views: 78
... and manipulated mainly at DNA level. 1 Plasmid vectors 2 Bacteriophage vectors 3 Cosmids 4 BACs & YACs Plasmid vectors Advantages: Small, ...
Date added: December 10, 2013 - Views: 15
Because the relative orientation of these cosmids is unknown, the gene order shown here is arbitrary along the chromosome but correct within the contig.
Date added: November 9, 2015 - Views: 1
BIOL 2416 Genetics Chapter 8: Mapping and Sequencing of Genomes
Date added: March 2, 2014 - Views: 18
... (via virus mechanisms); phage cosmids Cloning Step 3 ...vectors contain selectable markers, only cells that contain vector DNA will survive selection, ...
Date added: October 6, 2011 - Views: 158
Fundamentals of Mapping and Sequencing Basic principles and procedures of mapping and DNA sequencing
Date added: October 19, 2013 - Views: 11
Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid
Date added: May 2, 2013 - Views: 21
... (2 – 10 Kbp) cosmids (40 Kbp) known dist ~500 bp ~500 bp 1. Find overlapping reads 4. Derive consensus sequence ..ACGATTACAATAGGTT.. 2.
Date added: December 2, 2014 - Views: 1
Chapter 9 Genomics Mapping and characterizing whole genomes 14 and 16 February, 2005
Date added: October 10, 2015 - Views: 1