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Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...

http://faculty.smu.edu/borr/ClassNotes/10-19-05.ppt

Date added: August 2, 2013 - Views: 6

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PowerPoint Presentation

... Cosmids PACs BACs Bacteriophage Vectors Lambda: Each clone can accept inserts of ~15-20 kb. Replace part of l DNA that is not needed for replication with ...

http://faculty.smu.edu/borr/ClassNotes/10-17-05.ppt

Date added: January 4, 2015 - Views: 1

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PowerPoint Presentation

... screening labelling PowerPoint Presentation PowerPoint Presentation phage and cosmid screening phages cosmids PACs BACs PowerPoint Presentation YACs ...

http://genemol.org/molecular-bio/technics.ppt

Date added: October 12, 2011 - Views: 27

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PowerPoint Presentation

... cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation ...

http://people.cst.cmich.edu/schis1j/bio326-02/lectures/11-8.ppt

Date added: January 13, 2013 - Views: 22

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Cloning into Plasmids - Buffalo State College

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 104

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PowerPoint Presentation

... Life cycle of a bacteriophage 2-8 Constructing a genomic library in phage 2-9 Constructing a genomic library in cosmids 2-10 Constructing a genomic ...

http://www.pascack.k12.nj.us/cms/lib5/NJ01000238/Centricity/Domain/142/WSSP06-Ch2-LIbraries.ppt

Date added: August 8, 2014 - Views: 3

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Slide 1

BACTERIOPHAGE LAMBDA COSMID VECTOR Purpose: 1. Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202010%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: October 7, 2011 - Views: 455

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PowerPoint Presentation

Molecular Basis for Relationship between Genotype and Phenotype DNA genotype DNA sequence transcription RNA translation amino acid sequence protein function

http://public.gettysburg.edu/~hiraizum/bio211/0909.ppt

Date added: September 8, 2014 - Views: 2

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No Slide Title

... replicatioin from two different organisms Can be replicated in both Often used to shuttle plasmids from bacteria to Cosmids – related to bacteriophages ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: May 6, 2013 - Views: 18

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Biotechnology and Genetic Engineering-PBIO 450/550

Title: Biotechnology and Genetic Engineering-PBIO 450/550 Author: showalte Last modified by: Allan Showalter Created Date: 9/8/2003 3:48:03 PM Document presentation ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB7200%20Lecture%203.ppt

Date added: February 13, 2013 - Views: 52

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www.cell.com

... (DUP25) region showing STSs and genes surrounding the BACs and cosmids used as FISH probes (Gratacòs et al. 2001). The centromeric (CEN) and telomeric ...

http://www.cell.com/ajhg/ppt/S0002-9297(07)60569-2.ppt

Date added: August 22, 2015 - Views: 1

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DUAL TRAFFICKING PATHWAYS OF CONNEXINS TO GAP...

... Progress to date: 105 of 319 cosmids fully processed 11493 independent insertions 10459 insertions in 2520 orfs (of 7825 in total) 4.2 ...

http://www.swansea.ac.uk/media/media,35187,en.ppt

Date added: May 23, 2014 - Views: 7

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No Slide Title

... phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, cDNA Nucleic acid probes - cloned DNA or synthetic oligos “Instant” cloning ...

http://www.bio.utk.edu/humangenetics/lecture/molec_tools06sep02.ppt

Date added: March 7, 2015 - Views: 1

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Die Achterbahn - Swansea University

... in E. coli Recombinant cosmid-DNA can easily be confirmed by PCR, restriction analysis and/or sequencing Mutagenised cosmids can be mobilised by conjugation, ...

http://www.swansea.ac.uk/media/media,35188,en.ppt

Date added: May 15, 2013 - Views: 2

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No Slide Title

Cosmids combination of lambda phage and plasmid hold inserts 35-45 kb FIGURE 20.6 3. Artificial Chromosomes YAC (yeast artificial Chromosomes - 500 ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: March 14, 2012 - Views: 95

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Foundations of Biology - Geoscience Research...

... but not in the middle Cosmids A cosmid is a plasmid with the l phage cos site included in it The cos site signals for packaging into l phage, ...

http://grisda.org/resources/Power_Points/Molecular_Genetics/Fly_Genome.ppt

Date added: December 10, 2013 - Views: 3

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3 Basic Principles of Recombinant DNA Technology

3 Basic Principles of Recombinant DNA Technology. Brief Chapter Outline I. Cause for Concern? Recombinant DNA Technology: Promise and Controversy

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: May 26, 2012 - Views: 184

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PowerPoint Presentation

AD000092 /FEATURE=cds#1 /DEFINITION=CH19HHR23 Homo sapiens DNA from chromosome 19p13.2 cosmids R31240, R30272 and R28549 containing the EKLF, GCDH, CRTC, ...

http://combio.cs.brandeis.edu/hong/CS230a/CourseInfo/DetectDEG.ppt

Date added: June 22, 2012 - Views: 54

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Viral Vectors - homepage.usask.ca

Viral Vectors May 8 Viral vectors ... cell-cell spread suppression of class I MHC promoter pA pack HSV-ori bacterial plasmid seq cloning site defective virus cosmids ...

http://homepage.usask.ca/~vim458/vtmc831/May8.ppt

Date added: February 19, 2012 - Views: 48

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Basic Molecular Biology Information - NIU...

Title: Basic Molecular Biology Information Author: t80maj1 Last modified by: t80maj1 Created Date: 8/21/2006 4:36:06 PM Document presentation format

http://www.bios.niu.edu/johns/humgen/cancer.ppt

Date added: October 29, 2011 - Views: 82

ppt
Presentazione standard di PowerPoint - Unife

Cloning in Cosmids. Production of overlapping restriction fragments by partial digestion of human genomic DNA with Sau3A. CHROMOSOME WALKING. CHROMOSOME WALKING.

http://m.docente.unife.it/dario.balestra/tecnologie-ricombinanti-2015/5-genoteche-cdnateche.pptx

Date added: June 27, 2015 - Views: 1

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Udspaltning af den recessive q = 0,01 og p = 0,99...

Chapter 10: Chromosomes and chromosome errors Making chromosome preparations Normal karyotypes from domestic animals Chromosome errors Identification of chromosomes ...

http://www.ihh.kvl.dk/htm/kc/popgen/genetics/10/10/kap10e.PPT

Date added: May 3, 2013 - Views: 2

ppt
PowerPoint Presentation

cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be

http://genemol.org/molecular-bio/7-Artificial-chromosomes/YAC-BAC-PAC.ppt

Date added: October 7, 2011 - Views: 229

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PowerPoint Presentation

Title: PowerPoint Presentation Author: Wenun Last modified by: Wenun Created Date: 8/26/2004 6:56:18 AM Document presentation format: On-screen Show

http://mis.agri.cmu.ac.th/download/course/lec_359401_03%BE%D1%B9%B8%D8%C7%D4%C8%C7%A1%C3%C3%C1%C8%D2%CA%B5%C3%EC.ppt

Date added: May 31, 2013 - Views: 3

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Genética Molecular em Medicina Transfusional

Whole Genome Shotgun Sequencing cut many times at random genome forward-reverse linked reads plasmids (2 – 10 Kbp) cosmids (40 Kbp) ...

http://homepage.ufp.pt/jcabeda/slides/gmed/gmed_sequenciacao.ppt

Date added: August 3, 2013 - Views: 7

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Microarray-based Disease Prognosis using Gene...

Microarray-based Disease Prognosis using Gene Annotation Signatures Michael Kovshilovsky Swapna Annavarapu SoCalBSI 2005

http://instructional1.calstatela.edu/jmomand2/2005/presentations/ppt/annavarapu_kovshilovsky.ppt

Date added: August 24, 2014 - Views: 41

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Vectors - College of Computer, Mathematical, and...

Most vectors are genetically engineered plasmids or phages. There are also cosmid vectors, ... Cosmids combine essential elements of a plasmid and Lambda systems.

http://www.clfs.umd.edu/grad/mlfsc/res/RecombomamtDNA&Medicine.ppt

Date added: September 11, 2011 - Views: 283

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Plasmids and Vectors - Molecular Biology for...

Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...

https://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: January 31, 2015 - Views: 8

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justc/courses/RecombDNA/Week6. ppt - Portland...

Cosmids BACs PACs YACs Cosmids replicate as high copy number plasmids--tend to be unstable, ... So you follow the protocol for Or…buy a kit/premade library…

http://web.pdx.edu/~justc/courses/RecombDNA/Week6.ppt

Date added: October 7, 2011 - Views: 78

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Chapter 12

Plasmid Placement in Cell PowerPoint Presentation Phage vectors Phage Insertion Cosmids YACs YACs YACs Essential elements for YACs PowerPoint Presentation Particle ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: May 3, 2013 - Views: 37

ppt
PowerPoint Presentation

... of 2 million plus reads derived from low copy BACs defined by Dr. Tabata et al. 3) identification of low copy cosmids (US) ...

https://solgenomics.net/static_content/solanaceae-project/sol-bioinformatics/pag2007_docs/pag2007_jg_bacext.ppt

Date added: April 29, 2016 - Views: 1

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chapter_08.ppt - Department of Biology - College...

Fosmids offer higher stability than comparable high copy number cosmids. Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.

http://www.bio.miami.edu/mccracken/genetics/lectures-2013/chapter_08.ppt

Date added: October 2, 2014 - Views: 4

ppt
PowerPoint Presentation

Title: PowerPoint Presentation Author: Mike Bartlett Last modified by: Justin Courcelle Created Date: 3/31/2003 6:09:42 AM Document presentation format

http://web.pdx.edu/~justc/courses/RecombDNA/Week4.ppt

Date added: October 3, 2011 - Views: 111

ppt
PowerPoint Presentation

Insert size range < 15kb. Bacteriophage-based Cosmids: Linear DNA molecules used for genomic and cDNA cloning. Bacterial host. Insert size range < 20kb. ...

http://users.umassmed.edu/maria.zapp/Block2Lecture1%2011_01_04.ppt

Date added: October 7, 2011 - Views: 123

ppt
PowerPoint Presentation

Fundamentals of Mapping and Sequencing Basic principles and procedures of mapping and DNA sequencing

http://forest.mtu.edu/faculty/joshi/new/BL3300%202006/BL3300%202006%20slides/chapter%203/03%20091904%20cpj.ppt

Date added: October 19, 2013 - Views: 13

ppt
Slide 1

Mapping the Human Genome Genetic Mapping Physical Mapping DNA Sequencing

https://biosci-batzerlab.biology.lsu.edu/Hum_Mol_Gen_Lectures/Hum%20Mol%20Genet%20%20Lecture%203%20Feburary%204,%202003.ppt

Date added: October 22, 2015 - Views: 1

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www.cell.com

The cosmids from within the IGLL locus that are used for FISH are indicated on the top line over the region shaded in light red.

http://www.cell.com/ajhg/ppt/S0002-9297(07)62073-4.ppt

Date added: February 13, 2016 - Views: 1

ppt
PowerPoint Presentation - DNA Libraries

Title: PowerPoint Presentation - DNA Libraries Author: Jeffery Young Last modified by: Jeff Young Created Date: 10/23/2009 5:04:36 PM Document presentation format

http://www.biol.wwu.edu/young/321/lectures/lg8.ppt

Date added: October 6, 2011 - Views: 160

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Genetic Engineering - University of North Carolina...

Genetic Engineering ... Single Cleavage Site High copy Numbers Insertion Inactivation Virus Vectors Lambda Phage Vector Stable Packaging Cosmids Other Vectors ...

p://people.uncw.edu/sizemorer

Date added: November 5, 2014 - Views: 203

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Chapter 4 Molecular Cloning Methods - Xiamen...

Chapter 4 Molecular Cloning Methods ... Cosmids can accept even larger inserts—up to 50 kb—making them a favorite choice for genomic libraries.

http://yjsy.xmu.edu.cn/Cn/uploads/Chapter%204.ppt

Date added: January 28, 2012 - Views: 183

ppt
PowerPoint Presentation

... and manipulated mainly at DNA level. 1 Plasmid vectors 2 Bacteriophage vectors 3 Cosmids 4 BACs & YACs Plasmid vectors Advantages: Small, ...

http://faculty.ksu.edu.sa/73614/amustafa/355/chapter3.ppt

Date added: December 10, 2013 - Views: 15

ppt
PowerPoint Presentation

BIOL 2416 Genetics Chapter 8: Mapping and Sequencing of Genomes

http://m.learning.hccs.edu/faculty/jessica.moody/biol2416/power-point-presentations/chapter-8

Date added: March 2, 2014 - Views: 18

ppt
PowerPoint Presentation

... from GeneScan and Ensembl are occasionally contradictory chicken also contained small mini-chromosomes that resemble cosmids which were lumped into the ...

http://instructional1.calstatela.edu/jmomand2/2004/presentations/ppt/ng.ppt

Date added: May 6, 2013 - Views: 7

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Forward Genetics - University of Colorado Boulder

Forward Genetics Survey ... mor-2 sup-23 evl-7 Genetic map map unit Cloned genes Non-cloned genes mDf4 nDf41 rme-2 unc-5 fem-1 skn-1 Physical map cosmids YACs ...

http://www.colorado.edu/MCDB/HanLab/part2.baisc_forward_genetics.ppt

Date added: September 24, 2011 - Views: 17

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Part II: Recombinant DNA Technology.ppt - Rajesh...

Title: Part II: Recombinant DNA Technology.ppt Author: ITR Last modified by: Konkuk university Created Date: 1/20/2002 6:56:56 PM Document presentation format

http://www.rajeshbanu.com/ebt/molecular%20breeding_RDT_2.ppt

Date added: March 1, 2016 - Views: 1

ppt
Slide 1

Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid

http://ecourse.sl.on.ca/mjsibbald/mjsibbald/08mb/0807mbviral%20vectors%20and%20other%20vectors.ppt

Date added: May 2, 2013 - Views: 25

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Lambda Vectors and their replication - Biology

Lambda vectors and their replication Sonita Gafary Biochem 72020 Lambda was first discovered at the Pasteur Institute by Andre Lwoff when he observed strains of E. Coli.

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: September 30, 2013 - Views: 49

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Freeman 1e: How we got there - oakland.edu

Plasmid vectors containing the lambda cos sites are called cosmids, ... Freeman 1e: How we got there Author: Pearson Education Created Date: 7/21/2004 7:08:57 PM

http://www2.oakland.edu/biology/chaudhry/pics/10.BacterialGenetics.ppt

Date added: May 7, 2012 - Views: 20

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Recombinant DNA Technology for the non-science...

Cosmids (artificial constructions) A prokaryotic vector should: 1. Be capable ... Recombinant DNA Technology for the non-science major. Author: Janey Last modified by:

http://cogs.csustan.edu/~tom/bioinfo/ppt/Day3.ppt

Date added: October 16, 2012 - Views: 32