Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...
Date added: August 2, 2013 - Views: 6
... Cosmids PACs BACs Bacteriophage Vectors Lambda: Each clone can accept inserts of ~15-20 kb. Replace part of l DNA that is not needed for replication with ...
Date added: January 4, 2015 - Views: 1
... screening labelling PowerPoint Presentation PowerPoint Presentation phage and cosmid screening phages cosmids PACs BACs PowerPoint Presentation YACs ...
Date added: October 12, 2011 - Views: 24
... cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation ...
Date added: January 13, 2013 - Views: 22
... & Cosmids (plasmid + l cos site & can be pckged into phage capsid) Transgenic Plant: Vector = Agrobacterium tumefaciens plasmid called Ti ...
Date added: August 2, 2013 - Views: 15
Title: PowerPoint Presentation Author: Mike Bartlett Last modified by: Justin Courcelle Created Date: 3/31/2003 6:09:42 AM Document presentation format
Date added: October 3, 2011 - Views: 105
BACTERIOPHAGE LAMBDA COSMID VECTOR Purpose: 1. Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.
Date added: October 7, 2011 - Views: 450
... Life cycle of a bacteriophage 2-8 Constructing a genomic library in phage 2-9 Constructing a genomic library in cosmids 2-10 Constructing a genomic ...
Date added: August 8, 2014 - Views: 3
Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...
Date added: December 2, 2011 - Views: 101
Biotechnology and Genetic Engineering PBIO 450/550 Gene libraries cDNA libraries Library screening Eukaryotic gene organization enhancers silencers Genomic library ...
Date added: October 6, 2011 - Views: 28
Genome Sequencing Bacteriophage fX174, the first genome to be sequenced, is a viral genome with only 5,368 base pairs ... a set of cDNA sequences to a set of cosmids, ...
Date added: September 12, 2011 - Views: 73
Molecular Basis for Relationship between Genotype and Phenotype DNA genotype DNA sequence transcription RNA translation amino acid sequence protein function
Date added: September 8, 2014 - Views: 2
... phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, cDNA Nucleic acid probes - cloned DNA or synthetic oligos “Instant” cloning ...
Date added: November 28, 2013 - Views: 4
... in E. coli Recombinant cosmid-DNA can easily be confirmed by PCR, restriction analysis and/or sequencing Mutagenised cosmids can be mobilised by conjugation, ...
Date added: May 15, 2013 - Views: 2
... Progress to date: 105 of 319 cosmids fully processed 11493 independent insertions 10459 insertions in 2520 orfs (of 7825 in total) 4.2 ...
Date added: May 23, 2014 - Views: 7
Title: Biotechnology and Genetic Engineering-PBIO 450/550 Author: showalte Last modified by: Allan Showalter Created Date: 9/8/2003 3:48:03 PM Document presentation ...
Date added: February 13, 2013 - Views: 48
... replicatioin from two different organisms Can be replicated in both Often used to shuttle plasmids from bacteria to Cosmids – related to bacteriophages ...
Date added: May 6, 2013 - Views: 10
... (DUP25) region showing STSs and genes surrounding the BACs and cosmids used as FISH probes (Gratacòs et al. 2001). The centromeric (CEN) and telomeric ...
Date added: August 22, 2015 - Views: 1
Recombinant DNA Technology Needles in Haystacks How to find one gene in large genome? A gene might be 1/1,000,000 of the genome. Three basic approaches: 1.
Date added: October 7, 2011 - Views: 271
... but not in the middle Cosmids A cosmid is a plasmid with the l phage cos site included in it The cos site signals for packaging into l phage, ...
Date added: December 10, 2013 - Views: 3
... Cloning by cosmids. The cosmid is cut at a BglII site next to the cos site. Donor genomic DNA is cut using Sau3A, which gives sticky ends compatible with BglII.
Date added: April 27, 2015 - Views: 1
Cosmids combination of lambda phage and plasmid hold inserts 35-45 kb FIGURE 20.6 3. Artificial Chromosomes YAC (yeast artificial Chromosomes - 500 ...
Date added: March 14, 2012 - Views: 83
The location of the three cosmids used for fluorescence in situ hybridization is also shown. de Vries.Reduced DAZ gene copy number.Fertil Steril 2002.
Date added: January 31, 2016 - Views: 1
... are important for cyclization Phage l DNA replication Phage l cloning vectors Cosmids cos site Only head of DNA can be packaged if flanked by cos sites ...
Date added: August 2, 2013 - Views: 1
3 Basic Principles of Recombinant DNA Technology. Brief Chapter Outline I. Cause for Concern? Recombinant DNA Technology: Promise and Controversy
Date added: May 26, 2012 - Views: 176
Title: Basic Molecular Biology Information Author: t80maj1 Last modified by: t80maj1 Created Date: 8/21/2006 4:36:06 PM Document presentation format
Date added: October 29, 2011 - Views: 81
AD000092 /FEATURE=cds#1 /DEFINITION=CH19HHR23 Homo sapiens DNA from chromosome 19p13.2 cosmids R31240, R30272 and R28549 containing the EKLF, GCDH, CRTC, ...
Date added: June 22, 2012 - Views: 51
Chapter 10: Chromosomes and chromosome errors Making chromosome preparations Normal karyotypes from domestic animals Chromosome errors Identification of chromosomes ...
Date added: May 3, 2013 - Views: 2
Capturing User Intent for Information Retrieval Hien Nguyen University of Connecticut Major advisor: Dr. Eugene Santos Jr. (UCONN) Associate advisor: Dr. Robert ...
Date added: July 3, 2014 - Views: 2
Viral Vectors May 8 Viral vectors ... cell-cell spread suppression of class I MHC promoter pA pack HSV-ori bacterial plasmid seq cloning site defective virus cosmids ...
Date added: February 19, 2012 - Views: 44
Title: PowerPoint Presentation Author: Wenun Last modified by: Wenun Created Date: 8/26/2004 6:56:18 AM Document presentation format: On-screen Show
Date added: May 31, 2013 - Views: 2
Cloning in Cosmids. Production of overlapping restriction fragments by partial digestion of human genomic DNA with Sau3A. CHROMOSOME WALKING. CHROMOSOME WALKING.
Date added: July 19, 2015 - Views: 1
cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be
Date added: October 7, 2011 - Views: 229
Microarray-based Disease Prognosis using Gene Annotation Signatures Michael Kovshilovsky Swapna Annavarapu SoCalBSI 2005
Date added: August 24, 2014 - Views: 36
Whole Genome Shotgun Sequencing cut many times at random genome forward-reverse linked reads plasmids (2 – 10 Kbp) cosmids (40 Kbp) ...
Date added: August 3, 2013 - Views: 7
Most vectors are genetically engineered plasmids or phages. There are also cosmid vectors, ... Cosmids combine essential elements of a plasmid and Lambda systems.
Date added: September 11, 2011 - Views: 279
Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...
Date added: January 31, 2015 - Views: 7
Plasmid Placement in Cell PowerPoint Presentation Phage vectors Phage Insertion Cosmids YACs YACs YACs Essential elements for YACs PowerPoint Presentation Particle ...
Date added: May 3, 2013 - Views: 37
Cosmids BACs PACs YACs Cosmids replicate as high copy number plasmids--tend to be unstable, ... So you follow the protocol for Or…buy a kit/premade library…
Date added: October 7, 2011 - Views: 78
Fosmids offer higher stability than comparable high copy number cosmids. Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.
Date added: October 2, 2014 - Views: 4
Insert size range < 15kb. Bacteriophage-based Cosmids: Linear DNA molecules used for genomic and cDNA cloning. Bacterial host. Insert size range < 20kb. ...
Date added: October 7, 2011 - Views: 120
Chapter 4 Molecular Cloning Methods Introduction The significance of gene cloning To elucidate the structure and function of genes. i.e.: investigating hGH gene hGH ...
Date added: January 28, 2012 - Views: 179
Mapping the Human Genome Genetic Mapping Physical Mapping DNA Sequencing
Date added: September 17, 2014 - Views: 2
... and manipulated mainly at DNA level. 1 Plasmid vectors 2 Bacteriophage vectors 3 Cosmids 4 BACs & YACs Plasmid vectors Advantages: Small, ...
Date added: December 10, 2013 - Views: 15
The cosmids from within the IGLL locus that are used for FISH are indicated on the top line over the region shaded in light red.
Date added: February 13, 2016 - Views: 1
BIOL 2416 Genetics Chapter 8: Mapping and Sequencing of Genomes
Date added: March 2, 2014 - Views: 18
Title: Présentation PowerPoint Author: Gérard Last modified by: S.T. Cole Created Date: 12/8/2002 3:49:59 PM Document presentation format: Présentation à l'écran
Date added: November 14, 2011 - Views: 25
Title: PowerPoint Presentation - DNA Libraries Author: Jeffery Young Last modified by: Jeff Young Created Date: 10/23/2009 5:04:36 PM Document presentation format
Date added: October 6, 2011 - Views: 159
... (2 – 10 Kbp) cosmids (40 Kbp) known dist ~500 bp ~500 bp 1. Find overlapping reads 4. Derive consensus sequence ..ACGATTACAATAGGTT.. 2.
Date added: December 2, 2014 - Views: 1