Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...
Date added: December 2, 2011 - Views: 100
Recombinant DNA Cont. Plasmids can carry up to 20,000 bp of foreign DNA. Besides bacterial plasmids, some other cloning vectors include viruses, bacteria artificial ...
Date added: August 27, 2013 - Views: 43
Inserting genes into Plasmids. The real-life application of what we have just learnt, occurs when geneticists insert an animal or plant gene into plasmids.
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DNA Technology is the application of our learned properties of DNA. ... Characteristics of Plasmids as Cloning Vectors. Natural vectors . Useful cloning vectors .
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Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...
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3 Basic Principles of Recombinant DNA Technology. ... A. Bacterial Vectors. 1. Plasmids. 2. Bacteriophage. ... Plant Cloning Vectors. 4. Mammalian Cell Vectors.
Date added: May 26, 2012 - Views: 175
Title: Molecular Cloning Methods Author: Santa Monica College Last modified by: Santa Monica College Created Date: 10/16/2001 2:16:45 AM Document presentation format
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Molecular Genetics Techniques BIT 220 Chapter 20 What is Cloning? Recombinant DNA technologies Restriction Endonucleases Why use REs? Plasmids Cloning Vectors Plasmid ...
Date added: March 14, 2012 - Views: 83
Some of plasmid applications. Plasmids serve as important tools in genetics and ... 3 Molecular cloning. Plasmids used in genetic engineering are called . vectors.
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Isolation of Plasmid DNA June 21, 2007 Leeward Community College E. coli Chromosome Plasmids Plasmids • DNA molecules separate from chromosomal DNA • Self ...
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Restriction Endonucleases, Plasmids, and Transformation Restriction Endonucleases Enzymes purified from bacteria Protect bacterial cell from viruses by degrading ...
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Genetic Changes Genetic Engineering Discovery of Restriction Enzymes Specific Cuts Making Recombinant DNA Making Recombinant DNA Using Plasmids Cloning Vectors Slide ...
Date added: May 11, 2013 - Views: 25
Bacterial Plasmids. Bacterial cells may contain extra-chromosomal DNA called ... Multiple Cloning Sites. Many cloning vectors contain a multiple cloning site or ...
Date added: May 22, 2015 - Views: 1
... Cutting with EcoRI Cloning vectors can be: plasmids, bacteriophages, viruses, small artificial chromosomes Vectors have at least one unique cloning site: ...
Date added: October 7, 2011 - Views: 53
Cloning vectors are DNA molecules that are used to "transport ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from ...
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Chapter 13 An Introduction to Cloning and Recombinant DNA Clones Genetically identical organisms or molecules derived from a common ancestor Cloning Plants from ...
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Plasmid Engineering p. 1-2 Cloning Scheme Digest ... Introduction to Vectors ... Slide 2 Slide 3 Slide 4 Plasmids Vectors Plasmid Engineering ...
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DNA-based information technologies Understand basics steps/enzymes/features of DNA cloning Know main types of cloning vectors used - pros & cons of each
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Cloning Vectors Enable DNA molecules to be replicated inside host ... Types of cloning vectors Plasmids: Autonomously replicating circular DNA molecules. 2.
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4-Summary Basic Plasmid Cloning Vectors. pBR322 and the pUC plasmids. pBR322 has . 2 antibiotic resistance genes . Many unique restriction sites for inserting foreign DNA
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Recombinant DNA Technology ... Most cloning vectors have a multiple cloning site, ... Plasmids for cloning carry drug resistance genes that are used for selection.
Date added: October 7, 2011 - Views: 271
Biotechnology and Recombinant DNA Chapter 9 I. Learning Objectives Why genetic engineering? Cloning basics Beyond the basics Overview of DNA manipulation II.
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Bacterial plasmids are widely used as cloning vectors because they can be isolated from bacteria, manipulated to form recombinant plasmids by . in vitro.
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Vector Construction II Gateway cloning ... The Plant Cell, Vol. 21: 4018–4030, December 2009 Vectors derived from plant sequences Slide 33 Slide 34 Slide 35 ...
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DNA ligase splices pieces into plasmids or other cloning vectors How to form recombinant DNA?
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Chapter 9 Molecular Genetic Techniques and Genomics Presented by: Troy Coke What and Why? What?: A gene of interest is inserted into another organism, enabling it to ...
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Plasmids are classified 1. by their ability to be transferred to other bacteria. ... The Cadillac of Cloning Vectors. pBR322. Clone fragment in one antibiotic gene.
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Cloning vectors Eukaryotic gene ... Bacteria carrying recombinant plasmids therefore give rise to white colonies. pGEM-3Z Cloning foreign DNA into a plasmid vector ...
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Cloning vectors Eukaryotic gene ... The high copy number of pUC plasmids is a result of the lack of the rop gene and a single point mutation in rep of pMB1; (2) bla ...
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VOCABULARY for GENETIC TECHNOLOGY CLONING Produces an ... Plasmids Plasmids are small rings of DNA in bacteria ... Transgenic organisms Plasmids VECTORS ...
Date added: May 13, 2013 - Views: 22
The simplest types of vectors are plasmids whose DNA is double-stranded and circular. ... Cloning Vectors. Three types of vectors commonly used for cloning into E. coli:
Date added: October 31, 2014 - Views: 13
Cloning Vectors and Plasmids Recombinant DNA Transgenic An organism with one or more genes from another organism inserted into its genome Gene Knockout Genes that ...
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... Restriction enzymes Cloning Vectors Plasmids that can be modified to carry new genes Plasmids useful as cloning vectors must have a replicator (origin of ...
Date added: July 1, 2013 - Views: 4
Plasmids almost always carry one or more genes, ... Several eukaryotic viruses have been employed as cloning vectors for specialized applications: for example,
Date added: May 5, 2015 - Views: 2
Types of cloning vectors Plasmids: Autonomously replicating circular DNA molecules. 2. Bacteriophage: Small viruses that infect bacteria cells. Vectors for large DNA ...
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The cloning process (1 ... conjugation genes oriT: origin of transfer PLASMIDS: APPLICATIONS • Cloning vectors • Gene expression • Transposon delivery ...
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Why do we care about homologous recombination? ... Plasmids can be cloning vectors Ch 8 pg 175 Plasmid requirements in biotech PowerPoint Presentation ...
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The Techniques of Molecular Genetics ... Many useful cloning vectors were derived from plasmid pBR322 ... Contain components from phage chromosomes and plasmids ...
Date added: December 26, 2013 - Views: 19
Recombinant DNA Technology rDNA Technology Restriction Enzymes and DNA Ligase Plasmid Cloning Vectors Transformation of Bacteria Blotting Techniques Restriction ...
Date added: November 22, 2011 - Views: 61
Students can become confused by the details of gene cloning through misunderstanding this basic editing ... have plasmids that can be used as gene vectors, and.
Date added: January 19, 2016 - Views: 1
* Cloning vectors * Cloning vectors In gene ... most commonly used are plasmid cloning vectors. * Plasmids Extra-chromosomal DNA found in ... PowerPoint Presentation ...
Date added: January 6, 2013 - Views: 19
DNA Cloning. Most techniques for cloning make use of bacteria (E. coli) as they have small circular plasmids that are receptive for insertion of foreign DNA.
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Gene cloning. leads to the ... are often used as vectors. 0. ... These are organisms that have had foreign genes inserted into their genome through plasmids created ...
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Microbial Genetics WHY? terms Genotype ... steps in cloning Restriction-modification enzymes “shot gun” or PCR Ligase Recombination Plasmids (or phage)= vectors ...
Date added: May 5, 2013 - Views: 13
Molecular cloning resources and technologies ... “sticky” or blunt ends after cleavage Ligation and vectors - plasmids, phage, cosmids, BACs, YACs (Table ...
Date added: November 28, 2013 - Views: 4
DNA Technology Chapter 13 Lab Biology 1A Chapter 15 Lab biology 2b Chapter 13 Honors Biology 4A
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Genetic Recombination Vertical Binary fission Budding ... Insulin Hormones Plasmids DNA Replicate independently Genetic Vectors Small Stable Marker ...
Date added: November 29, 2011 - Views: 39
DNA Technology Overview DNA ... DNA vectors, and host organisms Either plasmids or bacteriophages can ... gene cloning results when the foreign genes replicate inside ...
Date added: October 30, 2011 - Views: 89