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Cloning into Plasmids - Buffalo State College

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Cloning into Plasmids - Buffalo State College

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 95

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No Slide Title

Molecular Genetics Techniques BIT 220 Chapter 20 What is Cloning? Recombinant DNA technologies Restriction Endonucleases Why use REs? Plasmids Cloning Vectors Plasmid ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: March 14, 2012 - Views: 78

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No Slide Title

Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: May 6, 2013 - Views: 5

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16.2 In vivo gene cloning – the use of vectors -...

Inserting genes into Plasmids. ... which of course can be isolated and then introduced into bacterial cells via vectors. ... 16.2 In vivo gene cloning – the use of ...

http://vbio.weebly.com/uploads/2/8/2/4/2824362/16.2_-_in_vivo_gene_cloning_-_the_use_of_vectors.pptx

Date added: June 16, 2012 - Views: 36

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Cloning - Sam Houston State University

Cloning(Part 1) Created by: Haley Vrazel. ... Plasmids and other types of cloning vectors were used by Human Genome Project researchers to copy genes and other pieces ...

http://www.shsu.edu/~agr_www/documents/CloningPart1PP.pptx

Date added: August 27, 2013 - Views: 30

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3 Basic Principles of Recombinant DNA Technology

3 Basic Principles of Recombinant DNA Technology. ... A. Bacterial Vectors. 1. Plasmids. 2. Bacteriophage. 3. ... Plant Cloning Vectors.

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: May 26, 2012 - Views: 169

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Vector Construction II - Department of Plant...

Lecture 11 Chapter 7 Vector Construction II Gateway cloning David Mann Example of a plant expression vector set pANIC Made for switchgrass transformation: BioEnergy ...

http://plantsciences.utk.edu/nstewart_353_ppt/Lecture%2011%20Vector%20Construction-II.ppt

Date added: October 3, 2011 - Views: 31

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Genetic Engineering - Fremont Union High School...

... bacteria Recombinant plasmids Plasmids can be modified in biological labs Modified plasmid = Recombinant plasmid Plasmids can be used as cloning vectors to ...

http://web.fuhsd.org/pamela_chow/apbio/lectures/Protein%20Synthesis/Genetic%20Engineering.ppt

Date added: April 1, 2013 - Views: 49

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Molecular Cloning Methods - Santa Monica College

Title: Molecular Cloning Methods Author: Santa Monica College Last modified by: Santa Monica College Created Date: 10/16/2001 2:16:45 AM Document presentation format

http://homepage.smc.edu/bober_mary/Bio%2022/ch%2018%20recombinant%20DNA%20technology%20%20lecture%20darwin%2006%20part%201%20and%20part%202.ppt

Date added: August 1, 2013 - Views: 1

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Recombinant DNA and Genetic Engineering -...

... Engineering Discovery of Restriction Enzymes Specific Cuts Making Recombinant DNA Making Recombinant DNA Using Plasmids Cloning Vectors Slide 10 Gene ...

http://faculty.salisbury.edu/%7Emaholland/101DNA.ppt

Date added: May 11, 2013 - Views: 17

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Plasmid - KSU

Some of plasmid applications. Plasmids serve as important tools in genetics and biochemistry labs, ... vectors. Vector Element ... DNA cloning using plasmid . Author:

http://fac.ksu.edu.sa/sites/default/files/plasmid_lab_.pptx

Date added: November 16, 2014 - Views: 3

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Chapter 9 – DNA-Based Information Technologies

... Cutting with EcoRI Cloning vectors can be: plasmids, bacteriophages, viruses, small artificial chromosomes Vectors have at least one unique cloning site: ...

http://ww2.valdosta.edu/~dgosnell/3601/May%202005/DNA-Based%20Information%20Technologies.ppt

Date added: May 3, 2013 - Views: 22

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Introduction to molecular cloning - ABE

Plasmids – used in cloning small segments of DNA (10-15 kb) ... Molecular Cloning Author: Lab Last modified by: Pierriden Perez Created Date: 6/9/2006 11:02:09 PM

http://abe.leeward.hawaii.edu/Summer07/Introduction%20to%20Molecular%20Cloning.ppt

Date added: November 5, 2011 - Views: 91

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Slide 1

Cloning vectors are DNA molecules that are used to "transport ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from ...

http://www.aun.edu.eg/molecular_biology/Procedure%20from%20gene%20to%20protein/2%20Cloning%20vectors.pptx

Date added: January 25, 2015 - Views: 2

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Plasmid Isolation

Isolation of Plasmid DNA June 21, 2007 Leeward Community College E. coli Chromosome Plasmids Plasmids • DNA molecules separate from chromosomal DNA • Self ...

http://site.iugaza.edu.ps/mzaid/files/2010/02/How-to-purify-plasmid-DNA-using-silica-based-columns1.ppt

Date added: April 21, 2012 - Views: 6

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Chapter 13 An Introduction to Cloning and...

Chapter 13 An Introduction to Cloning and Recombinant DNA Clones Genetically identical organisms or molecules derived from a common ancestor Cloning Plants from ...

http://www.bio.brandeis.edu/classes/heredity/Lecture%20Powerpoints/Chapter_13_1.ppt

Date added: August 2, 2013 - Views: 19

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Introduction to Vectors - The Pingry School

Some plasmids are present in E. coli at 200-500 ... 2 Slide 3 Slide 4 Plasmids Vectors Plasmid Engineering Cloning Scheme Slide 9 Slide 10 DNA ...

http://faculty.pingry.org/domara/documents/WAKSMANLECTURE1_sept09.ppt

Date added: March 20, 2015 - Views: 5

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PowerPoint Presentation

DNA-based information technologies Understand basics steps/enzymes/features of DNA cloning Know main types of cloning vectors used - pros & cons of each

http://chemistry.creighton.edu/%7Ejksoukup/DNAtech2_2010.ppt

Date added: February 27, 2014 - Views: 18

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Ch. 9: Presentation Slides - Tistory

The simplest types of vectors are plasmids whose DNA is double-stranded and circular. ... Cloning Vectors. Three types of vectors commonly used for cloning into E. coli:

http://www.pnumbl.tistory.com/attachment/[email protected]

Date added: October 31, 2014 - Views: 3

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Library construction - Center for Bioinformatics...

A plasmid vector for cloning Plasmid vectors Expression vector How to clone DNA ... Blue/White Color Screening Selecting Colonies with Recombinant Plasmids ...

http://www.cbcb.umd.edu/confcour/CMSC828H-materials/Cloning-into-plasmids.ppt

Date added: February 12, 2013 - Views: 4

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Inquiry into Life Twelfth Edition - Regis...

4-Summary Basic Plasmid Cloning Vectors. pBR322 and the pUC plasmids. pBR322 has . 2 antibiotic resistance genes . Many unique restriction sites for inserting foreign DNA

http://academic.regis.edu/jbetz/BL426Lectures-Notes/chapt04%202011.pps

Date added: August 2, 2013 - Views: 38

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PowerPoint Presentation

Cloning Vectors Enable DNA molecules to be replicated inside host ... Types of cloning vectors Plasmids: Autonomously replicating circular DNA molecules. 2.

http://faculty.smu.edu/borr/ClassNotes/10-14-05.ppt

Date added: May 13, 2013 - Views: 26

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Ch. 9: Presentation Slides - Tistory

The simplest types of vectors are plasmids whose DNA is double-stranded and circular. When the DNA fragment has been joined to the vector, ... Cloning Vectors: BACs.

http://www.pnumbl.tistory.com/attachment/[email protected]

Date added: June 9, 2015 - Views: 1

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Biotechnology and Recombinant DNA

Biotechnology and Recombinant DNA Chapter 9 I. Learning Objectives Why genetic engineering? Cloning basics Beyond the basics Overview of DNA manipulation II.

http://wolfweb.unr.edu/homepage/borgeson/Bio251/10biotechnewa.ppt

Date added: July 28, 2013 - Views: 31

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Biotechnology and Genetic Engineering-PBIO 450/550

Cloning vectors Eukaryotic gene ... The high copy number of pUC plasmids is a result of the lack of the rop gene and a single point mutation in rep of pMB1; (2) bla ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB720Lecture211.ppt

Date added: May 14, 2013 - Views: 10

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PowerPoint Presentation

DNA ligase splices pieces into plasmids or other cloning vectors How to form recombinant DNA?

http://skywalker.cochise.edu/ashraft/web/BIO101ppt/What%20is%20biotechnology.ppt

Date added: November 8, 2014 - Views: 3

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Recombinant DNA

Plasmid vectors MIT Biology ... Benefits Disadvantages Works Cited “Cloning Genes ... DNA and Elements Utilizing Recombinant DNA Such as Plasmids and Viral ...

http://www.aryansexport.com/PPT/Recombinant%20DNA%20technology.ppt

Date added: June 26, 2014 - Views: 11

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Biotechnology and Genetic Engineering-PBIO 450/550

Cloning vectors Eukaryotic gene ... Bacteria carrying recombinant plasmids therefore give rise to white colonies. pGEM-3Z Cloning foreign DNA into a plasmid vector ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB720Lecture209.ppt

Date added: May 20, 2013 - Views: 5

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PowerPoint Presentation

Bacteria contain natural plasmids and viruses which are useful vectors for recombinant DNA. ... Plasmid Cloning Vectors. Small circular piece of extrachromosomalDNA.

http://m.docente.unife.it/dario.balestra/tecnologie-ricombinanti-2015/4-plasmidi.pptx

Date added: August 16, 2015 - Views: 1

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VOCABULARY for GENETIC TECHNOLOGY

VOCABULARY for GENETIC TECHNOLOGY CLONING Produces an EXACT COPY of organism; ... GENE SPLICING Next word: Transgenic organisms Plasmids VECTORS ...

http://www.bville.org/tfiles/folder196/VOCABULARY%20for%20GENETIC%20TECHNOLOGY%2Eppt

Date added: May 13, 2013 - Views: 14

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DNA Technology - Monroe Township School District

DNA Technology Chapter ... Recognizes CTTAAG * * Cloning Vectors Cloning vector is a carrier that is used ... plasmids are used to transfer a gene to ...

http://www.monroe.k12.nj.us/cms/lib010/NJ01000268/Centricity/Domain/270/DNA%20Technology.ppt

Date added: April 11, 2015 - Views: 5

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PowerPoint Presentation

The cloning process (1 ... conjugation genes oriT: origin of transfer PLASMIDS: APPLICATIONS • Cloning vectors • Gene expression • Transposon delivery ...

http://www.ibt.unam.mx/computo/pdfs/canovas/dos.ppt

Date added: February 11, 2013 - Views: 24

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No Slide Title

... Restriction enzymes Cloning Vectors Plasmids that can be modified to carry new genes Plasmids useful as cloning vectors must have a replicator (origin of ...

http://faperta.ugm.ac.id/download/pak_tar/genetika-molekuler/cloning20061.ppt

Date added: July 1, 2013 - Views: 2

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Genetic Engineering - WC Ag Ed

... Endonucleases Cloning Vectors Promoter ... and Environment Genetic Engineering Endonucleases Cloning Vectors ... Plasmids back to ...

http://lr010.k12.sd.us/Genetic%20Engineering%20Ch11.ppt

Date added: December 16, 2011 - Views: 114

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No Slide Title

Cloning Vectors and Plasmids Recombinant DNA Transgenic An organism with one or more genes from another organism inserted into its genome Gene Knockout Genes that ...

http://www.lake.k12.fl.us/cms/lib05/FL01000799/Centricity/Domain/2927/Ch_9-4_Genetic_Engineering.ppt

Date added: July 11, 2015 - Views: 1

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PowerPoint Presentation

* Cloning vectors * Cloning vectors In gene ... most commonly used are plasmid cloning vectors. * Plasmids Extra-chromosomal DNA found in ... PowerPoint Presentation ...

http://www.educationscotland.gov.uk/Images/Background_Transformation_PowerPoint_H_tcm4-664851.ppt

Date added: January 6, 2013 - Views: 11

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Chapter 9 Molecular Genetic Techniques and...

Chapter 9 Molecular Genetic Techniques and Genomics Presented by: Troy Coke What and Why? What?: A gene of interest is inserted into another organism, enabling it to ...

http://biology.uco.edu/bidlack/cellphys/doc/powerpoint%20s2004/Recombinant%20DNA.ppt

Date added: August 2, 2013 - Views: 5

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Chapter 15 The Techniques of Molecular Genetics

The Techniques of Molecular Genetics ... Many useful cloning vectors were derived from plasmid pBR322 ... Contain components from phage chromosomes and plasmids ...

http://www2.fiu.edu/~barbieri/CH14.ppt

Date added: December 26, 2013 - Views: 14

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VIRAL VECTORS IN GENE THERAPY.ppt -...

VIRAL VECTORS IN GENE THERAPY Gene Therapy The introduction of nucleic acids into cells for the purpose of altering the course of medical condition or disease.

http://www.rci.rutgers.edu/~bhillman/comparative_virology/NT-GeneTherapy2005.ppt

Date added: November 20, 2011 - Views: 79

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Bacterial recombination- Transduction

Why do we care about homologous recombination? ... Plasmids can be cloning vectors Ch 8 pg 175 Plasmid requirements in biotech PowerPoint Presentation ...

http://www.mccc.edu/~blinderl/documents/Ch15_bacteriaslide_set2.ppt

Date added: March 29, 2015 - Views: 3

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DNA Technology & Gene Mapping

DNA Cloning. Most techniques for cloning make use of bacteria (E. coli) as they have small circular plasmids that are receptive for insertion of foreign DNA.

http://www.mrkelly.santasusana.org/DNA%20Technology%20&%20Gene%20Mapping.pptx

Date added: August 16, 2015 - Views: 1

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What is gene clonning? - FaPGenT --- Fatih...

Plasmids almost always carry one or more genes, ... Several eukaryotic viruses have been employed as cloning vectors for specialized applications: for example,

http://www.fapgent.org/uploads/1/8/9/7/18975273/cpt_1-2.pptx

Date added: May 5, 2015 - Views: 1

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Microbial Genetics - ALRlab

Microbial Genetics WHY? terms Genotype ... steps in cloning Restriction-modification enzymes “shot gun” or PCR Ligase Recombination Plasmids (or phage)= vectors ...

http://alrlab.pdx.edu/media/Lec12.ppt

Date added: May 5, 2013 - Views: 11

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RNA interference as a resistance mechanism against...

Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning ...

http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03977.x/figure.pptx?figureAssetHref=image_n/JAM_3977_f3.gif

Date added: May 27, 2015 - Views: 1

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Chapter 3 Methods in Molecular Biology and Genetic...

Blue/white selection allows the identification of bacteria that contain the vector plasmid and vector plasmids ... Several types of cloning vectors are available ...

http://teacherweb.com/CA/SolanoCommunityCollege/EdwardRe/XXXXX-Biosci-2-Spring-2015-Introduction-to-Molecular-Biology-XXXXX.ppt

Date added: March 29, 2015 - Views: 1

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DNA Technology - NCSSM Links

... DNA vectors, and host organisms Either plasmids or bacteriophages can serve as ... gene cloning results when the foreign genes replicate inside the host bacterium ...

http://courses.ncssm.edu/mccoy/DNA%20Technology.ppt

Date added: October 30, 2011 - Views: 82

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PowerPoint Presentation

Plasmids are also known as cloning vectors. Cloning Vector – a DNA molecule that can carry foreign DNA into a host cell and replicate there. Examples of Gene Cloning.

http://intranet.oswego308.org/schools/uploads/files/70491/Chapter%2020%20-%20Biotechnology.pptx

Date added: May 25, 2014 - Views: 1

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Ligation and Transformation - Plant Genome...

... amplification of gene product A type of cloning vector used to ... of vectors GFP keeps its ... Picture Transformation Plasmids Overall ...

http://www.plantgenomeoutreach.eeob.iastate.edu/teachmod/Bacterial_Transformation.ppt

Date added: October 14, 2011 - Views: 64

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DNA Cloning Yields multiple copies of a gene or...

Plasmids are also known as cloning vectors. Cloning Vector – a DNA molecule that can carry foreign DNA into a host cell and replicate there. Examples of Gene Cloning.

http://intranet.oswego308.org/schools/uploads/files/70491/Chapter%2020%20-%20Biotechnology(1).pptx

Date added: May 1, 2015 - Views: 1