Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...
Date added: December 2, 2011 - Views: 95
Recombinant DNA Cont. Plasmids can carry up to 20,000 bp of foreign DNA. Besides bacterial plasmids, some other cloning vectors include viruses, bacteria artificial ...
Date added: August 27, 2013 - Views: 39
Inserting genes into Plasmids. ... which of course can be isolated and then introduced into bacterial cells via vectors. ... 16.2 In vivo gene cloning – the use of ...
Date added: June 16, 2012 - Views: 38
Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...
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Molecular Genetics Techniques BIT 220 Chapter 20 What is Cloning? Recombinant DNA technologies Restriction Endonucleases Why use REs? Plasmids Cloning Vectors Plasmid ...
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3 Basic Principles of Recombinant DNA Technology. ... A. Bacterial Vectors. 1. Plasmids. 2. Bacteriophage. ... Plant Cloning Vectors. 4. Mammalian Cell Vectors.
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3 Molecular cloning. Plasmids used in genetic engineering are called . vectors. Vector Element . Vector Element . Vector Element .
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Cloning vectors are DNA molecules that are used to "transport ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from ...
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Genetic Changes Genetic Engineering Discovery of Restriction Enzymes Specific Cuts Making Recombinant DNA Making Recombinant DNA Using Plasmids Cloning Vectors Slide ...
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Restriction Endonucleases, Plasmids, and Transformation Restriction Endonucleases Enzymes purified from bacteria Protect bacterial cell from viruses by degrading ...
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Title: Molecular Cloning Methods Author: Santa Monica College Last modified by: Santa Monica College Created Date: 10/16/2001 2:16:45 AM Document presentation format
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Chapter 9 – DNA-Based Information Technologies Recombinant DNA molecules are constructed with DNA from different sources Recombinant DNA molecules are created often ...
Date added: May 3, 2013 - Views: 22
Genetic Engineering = inserting a foreign gene of ... plasmid = Recombinant plasmid Plasmids can be used as cloning vectors to get the recombinant plasmid ...
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Chapter 13 An Introduction to Cloning and Recombinant DNA Clones Genetically identical organisms or molecules derived from a common ancestor Cloning Plants from ...
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Types of Cloning Vectors. A. Plasmid Vectors. Plasmids are relatively small, circular DNA molecules. They are commonly used as vectors for DNA fragments up to
Date added: February 12, 2015 - Views: 5
DNA-based information technologies Understand basics steps/enzymes/features of DNA cloning Know main types of cloning vectors used - pros & cons of each
Date added: February 27, 2014 - Views: 18
Some plasmids are present in E. coli at 200-500 ... 2 Slide 3 Slide 4 Plasmids Vectors Plasmid Engineering Cloning Scheme Slide 9 Slide 10 DNA ...
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A plasmid vector for cloning Plasmid vectors Expression vector How to clone DNA ... Blue/White Color Screening Selecting Colonies with Recombinant Plasmids ...
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4-Summary Basic Plasmid Cloning Vectors. pBR322 and the pUC plasmids. pBR322 has . 2 antibiotic resistance genes . Many unique restriction sites for inserting foreign DNA
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Cloning Vectors Enable DNA molecules to be replicated inside host ... Types of cloning vectors Plasmids: Autonomously replicating circular DNA molecules. 2.
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TOPO cloning is the enzyme DNA topoisomeraseI, ... TOPO vectors are provided linearized with topoisomerase I covalently bound to each 3´ phosphate.
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Benefits Disadvantages Works Cited “Cloning ... Fact Sheet Describing Recombinant DNA and Elements Utilizing Recombinant DNA Such as Plasmids and Viral Vectors, ...
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Lecture 11 Chapter 7 Vector Construction II Gateway cloning David Mann Example of a plant expression vector set pANIC Made for switchgrass transformation: BioEnergy ...
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DNA ligase splices pieces into plasmids or other cloning vectors How to form recombinant DNA?
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Biotechnology and Recombinant DNA Chapter 9 I. Learning Objectives Why genetic engineering? Cloning basics Beyond the basics Overview of DNA manipulation II.
Date added: July 28, 2013 - Views: 36
The simplest types of vectors are plasmids whose DNA is double-stranded and circular. ... Cloning Vectors. Three types of vectors commonly used for cloning into E. coli:
Date added: October 31, 2014 - Views: 6
Cloning vectors Eukaryotic gene ... Bacteria carrying recombinant plasmids therefore give rise to white colonies. pGEM-3Z Cloning foreign DNA into a plasmid vector ...
Date added: October 6, 2011 - Views: 45
Cloning vectors Eukaryotic gene ... The high copy number of pUC plasmids is a result of the lack of the rop gene and a single point mutation in rep of pMB1; (2) bla ...
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VOCABULARY for GENETIC TECHNOLOGY CLONING Produces an ... Plasmids Plasmids are small rings of DNA in bacteria ... Transgenic organisms Plasmids VECTORS ...
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Plasmids are classified 1. by their ability to be transferred to other bacteria. ... The Cadillac of Cloning Vectors. pBR322. Clone fragment in one antibiotic gene.
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Chapter 9 Molecular Genetic Techniques and Genomics Presented by: Troy Coke What and Why? What?: A gene of interest is inserted into another organism, enabling it to ...
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Cloning Vectors and Plasmids Recombinant DNA Transgenic An organism with one or more genes from another organism inserted into its genome Gene Knockout Genes that ...
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Cloning and Expression of ... up foreign DNA Selecting Cells with Desired Gene Most cells don’t take up DNA Ampicillin resistance gene in plasmid vectors Only cells ...
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... Restriction enzymes Cloning Vectors Plasmids that can be modified to carry new genes Plasmids useful as cloning vectors must have a replicator (origin of ...
Date added: July 1, 2013 - Views: 2
Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning ...
Date added: May 27, 2015 - Views: 1
All cloning vectors systems did not produce recombinant libraries with colony frequencies that mirror the original sample ... Plasmids can be shuttle vectors, ...
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... (phage and plasmids) ... different cloning vectors must be used in different species. Special cloning vectors can replicate in other prokaryotes and in eukaryotes ...
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The cloning process (1 ... conjugation genes oriT: origin of transfer PLASMIDS: APPLICATIONS • Cloning vectors • Gene expression • Transposon delivery ...
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Why do we care about homologous recombination? ... Plasmids can be cloning vectors Ch 8 pg 175 Plasmid requirements in biotech PowerPoint Presentation ...
Date added: March 29, 2015 - Views: 3
* Cloning vectors * Cloning vectors In gene ... most commonly used are plasmid cloning vectors. * Plasmids Extra-chromosomal DNA found in ... PowerPoint Presentation ...
Date added: January 6, 2013 - Views: 11
Plasmids are also known as cloning vectors. Cloning Vector – a DNA molecule that can carry foreign DNA into a host cell and replicate there. Examples of Gene Cloning.
Date added: May 1, 2015 - Views: 1
Bacterial Transformation ... A type of cloning vector used to carry a gene ... Overall Picture Transformation Plasmids Overall Transformation ...
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Microbial Genetics WHY? terms Genotype refers to genetic makeup Phenotype refers to expression of that genetic makeup Heritable traits must be encoded in DNA
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DNA Technology Overview DNA ... DNA vectors, and host organisms Either plasmids or bacteriophages can ... gene cloning results when the foreign genes replicate inside ...
Date added: October 30, 2011 - Views: 82
Chapter 7 Recombinant DNA Technology and Genomics
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Cloning Vectors. name given original plasmid. dfn: ... large plasmids trimmed down so they contain just the genes necessary for replication. carry 100 – 300 kb ...
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Frameshift mutations A single base-pair deletion or insertion results in a change in the reading frame AUG UUU AGC UUU AGC UUU AGC WT Met Phe Ser Phe Ser Phe Ser
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Plasmids (small, circular DNA molecules independent of the bacterial chromosome) are often used as vectors. 0. ... Figure 12.1 An overview of gene cloning.
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