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Recombinant DNA - Egg Harbor Township Schools

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Recombinant DNA - Egg Harbor Township Schools

DNA cloning yields multiple copies of a gene of other DNA segment-Vocab. ... Vectors are often Plasmids. What is a plasmid? Circular, autonomous molecules of DNA.

http://www.eht.k12.nj.us/~oneilj/NEURO_GENETICS_files/Genetics/Recombinant%20DNA_cloning_plasmids_vectors.pptx

Date added: April 17, 2014 - Views: 14

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Cloning into Plasmids - Buffalo State College

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 95

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Cloning - Sam Houston State University

Recombinant DNA Cont. Plasmids can carry up to 20,000 bp of foreign DNA. Besides bacterial plasmids, some other cloning vectors include viruses, bacteria artificial ...

http://www.shsu.edu/~agr_www/documents/CloningPart1PP.pptx

Date added: August 27, 2013 - Views: 30

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16.2 In vivo gene cloning – the use of vectors -...

Inserting genes into Plasmids. The real-life application of what we have just learnt, occurs when geneticists insert an animal or plant gene into plasmids.

http://vbio.weebly.com/uploads/2/8/2/4/2824362/16.2_-_in_vivo_gene_cloning_-_the_use_of_vectors.pptx

Date added: June 16, 2012 - Views: 36

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3 Basic Principles of Recombinant DNA Technology

3 Basic Principles of Recombinant DNA Technology. ... A. Bacterial Vectors. 1. Plasmids. 2. Bacteriophage. ... Plant Cloning Vectors. 4. Mammalian Cell Vectors.

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: May 26, 2012 - Views: 169

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No Slide Title

Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: May 6, 2013 - Views: 5

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No Slide Title

Molecular Genetics Techniques BIT 220 Chapter 20 What is Cloning? Recombinant DNA technologies Restriction Endonucleases Why use REs? Plasmids Cloning Vectors Plasmid ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: March 14, 2012 - Views: 78

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Slide 1

Cloning vectors are DNA molecules that are used to "transport ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from ...

http://www.aun.edu.eg/molecular_biology/Procedure%20from%20gene%20to%20protein/2%20Cloning%20vectors.pptx

Date added: January 25, 2015 - Views: 1

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Chapter 9 – DNA-Based Information Technologies

... Cutting with EcoRI Cloning vectors can be: plasmids, bacteriophages, viruses, small artificial chromosomes Vectors have at least one unique cloning site: ...

http://ww2.valdosta.edu/~dgosnell/3601/May%202005/DNA-Based%20Information%20Technologies.ppt

Date added: May 3, 2013 - Views: 21

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Chapter 13 An Introduction to Cloning and...

Chapter 13 An Introduction to Cloning and Recombinant DNA Clones Genetically identical organisms or molecules derived from a common ancestor Cloning Plants from ...

http://www.bio.brandeis.edu/classes/heredity/Lecture%20Powerpoints/Chapter_13_1.ppt

Date added: August 2, 2013 - Views: 19

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Recombinant DNA and Genetic Engineering -...

... Engineering Discovery of Restriction Enzymes Specific Cuts Making Recombinant DNA Making Recombinant DNA Using Plasmids Cloning Vectors Slide 10 Gene ...

http://faculty.salisbury.edu/%7Emaholland/101DNA.ppt

Date added: May 11, 2013 - Views: 17

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Biotechnology and Recombinant DNA

Biotechnology and Recombinant DNA Chapter 9 I. Learning Objectives Why genetic engineering? Cloning basics Beyond the basics Overview of DNA manipulation II.

http://wolfweb.unr.edu/homepage/borgeson/Bio251/10biotechnewa.ppt

Date added: July 28, 2013 - Views: 31

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Molecular Cloning Methods

Title: Molecular Cloning Methods Author: Santa Monica College Last modified by: Santa Monica College Created Date: 10/16/2001 2:16:45 AM Document presentation format

http://homepage.smc.edu/bober_mary/Bio%2022/ch%2018%20recombinant%20DNA%20technology%20%20lecture%20darwin%2006%20part%201%20and%20part%202.ppt

Date added: August 1, 2013 - Views: 1

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Introduction to Vectors - The Pingry School

Some plasmids are present in E. coli at 200-500 ... 2 Slide 3 Slide 4 Plasmids Vectors Plasmid Engineering Cloning Scheme Slide 9 Slide 10 DNA ...

http://faculty.pingry.org/domara/documents/WAKSMANLECTURE1_sept09.ppt

Date added: March 20, 2015 - Views: 5

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Recombinant DNA

Benefits Disadvantages Works Cited “Cloning ... Fact Sheet Describing Recombinant DNA and Elements Utilizing Recombinant DNA Such as Plasmids and Viral Vectors, ...

http://www.aryansexport.com/PPT/Recombinant%20DNA%20technology.ppt

Date added: June 26, 2014 - Views: 10

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Plasmid - KSU

Some of plasmid applications. Plasmids serve as important tools in genetics and biochemistry labs, ... vectors. Vector Element ... DNA cloning using plasmid . Author:

http://fac.ksu.edu.sa/sites/default/files/plasmid_lab_.pptx

Date added: November 16, 2014 - Views: 3

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PowerPoint Presentation

Plasmids are classified 1. by their ability to be transferred to other bacteria. ... The Cadillac of Cloning Vectors. pBR322. Clone fragment in one antibiotic gene.

http://m.docente.unife.it/dario.balestra/tecnologie-ricombinanti-2015/4-plasmidi.pptx/at_download/file

Date added: July 5, 2015 - Views: 1

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PowerPoint Presentation

Cloning Vectors Enable DNA molecules to be replicated inside host ... Types of cloning vectors Plasmids: Autonomously replicating circular DNA molecules. 2.

http://faculty.smu.edu/borr/ClassNotes/10-14-05.ppt

Date added: May 13, 2013 - Views: 26

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DNA Technology - Monroe Township School District

DNA Technology Chapter ... Recognizes CTTAAG * * Cloning Vectors Cloning vector is a carrier that is used ... plasmids are used to transfer a gene to ...

http://www.monroe.k12.nj.us/cms/lib010/NJ01000268/Centricity/Domain/270/DNA%20Technology.ppt

Date added: April 11, 2015 - Views: 3

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PowerPoint Presentation

DNA-based information technologies Understand basics steps/enzymes/features of DNA cloning Know main types of cloning vectors used - pros & cons of each

http://chemistry.creighton.edu/%7Ejksoukup/DNAtech2_2010.ppt

Date added: February 27, 2014 - Views: 18

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Introduction to molecular cloning - ABE

Plasmids – used in cloning small segments of DNA (10-15 kb) ... Molecular Cloning Author: Lab Last modified by: Pierriden Perez Created Date: 6/9/2006 11:02:09 PM

http://abe.leeward.hawaii.edu/Summer07/Introduction%20to%20Molecular%20Cloning.ppt

Date added: November 5, 2011 - Views: 89

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Inquiry into Life Twelfth Edition - Regis...

4-Summary Basic Plasmid Cloning Vectors. pBR322 and the pUC plasmids. pBR322 has . 2 antibiotic resistance genes . Many unique restriction sites for inserting foreign DNA

http://academic.regis.edu/jbetz/BL426Lectures-Notes/chapt04%202011.pps

Date added: August 2, 2013 - Views: 38

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Biotechnology and Genetic Engineering-PBIO 450/550

Cloning vectors Eukaryotic gene ... high copy number, E.coli plasmids, 2686 bp in length. ... Biotechnology and Genetic Engineering-PBIO 450/550 Author: showalte

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO450Lecture208.ppt

Date added: October 6, 2011 - Views: 41

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Library construction - Center for Bioinformatics...

A plasmid vector for cloning Plasmid vectors Expression vector How to clone DNA ... Blue/White Color Screening Selecting Colonies with Recombinant Plasmids ...

http://www.cbcb.umd.edu/confcour/CMSC828H-materials/Cloning-into-plasmids.ppt

Date added: February 12, 2013 - Views: 4

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VOCABULARY for GENETIC TECHNOLOGY

VOCABULARY for GENETIC TECHNOLOGY CLONING Produces an EXACT COPY of organism; ... GENE SPLICING Next word: Transgenic organisms Plasmids VECTORS ...

http://www.bville.org/tfiles/folder196/VOCABULARY%20for%20GENETIC%20TECHNOLOGY%2Eppt

Date added: May 13, 2013 - Views: 11

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Biotechnology and Genetic Engineering-PBIO 450/550

Complementary DNA or cDNA cloning: cDNA library construction ... How to make and screen cDNA libraries Several different cloning vectors including plasmids, ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB7200%20Lecture%203.ppt

Date added: February 13, 2013 - Views: 41

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PowerPoint Presentation

BACs are maintained as single copy plasmids in E. coli and exclude other BAC plasmids from ... pBeloBAC11 represents the second generation BAC cloning vectors.

http://genemol.org/molecular-bio/7-Artificial-chromosomes/YAC-BAC-PAC.ppt

Date added: October 7, 2011 - Views: 227

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Chapter 9 Molecular Genetic Techniques and...

Chapter 9 Molecular Genetic Techniques and Genomics Presented by: Troy Coke What and Why? What?: A gene of interest is inserted into another organism, enabling it to ...

http://biology.uco.edu/bidlack/cellphys/doc/powerpoint%20s2004/Recombinant%20DNA.ppt

Date added: August 2, 2013 - Views: 5

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DNA Technology - NCSSM Links

... DNA vectors, and host organisms Either plasmids or bacteriophages can serve as ... gene cloning results when the foreign genes replicate inside the host bacterium ...

http://courses.ncssm.edu/mccoy/DNA%20Technology.ppt

Date added: October 30, 2011 - Views: 82

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Bacterial recombination- Transduction

Why do we care about homologous recombination? ... Plasmids can be cloning vectors Ch 8 pg 175 Plasmid requirements in biotech PowerPoint Presentation ...

http://www.mccc.edu/~blinderl/documents/Ch15_bacteriaslide_set2.ppt

Date added: March 29, 2015 - Views: 3

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What is gene clonning? - FaPGenT --- Fatih...

Plasmids almost always carry one or more genes, ... Several eukaryotic viruses have been employed as cloning vectors for specialized applications: for example,

http://www.fapgent.org/uploads/1/8/9/7/18975273/cpt_1-2.pptx

Date added: May 5, 2015 - Views: 1

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Ch. 9: Presentation Slides - Tistory

The simplest types of vectors are plasmids whose DNA is double-stranded and circular. ... Cloning Vectors. Three types of vectors commonly used for cloning into E. coli:

http://www.pnumbl.tistory.com/attachment/[email protected]

Date added: October 31, 2014 - Views: 3

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PowerPoint Presentation

* Cloning vectors * Cloning vectors In gene ... most commonly used are plasmid cloning vectors. * Plasmids Extra-chromosomal DNA found in ... PowerPoint Presentation ...

http://www.educationscotland.gov.uk/Images/Background_Transformation_PowerPoint_H_tcm4-664851.ppt

Date added: January 6, 2013 - Views: 11

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Chapter 3 Methods in Molecular Biology and Genetic...

Blue/white selection allows the identification of bacteria that contain the vector plasmid and vector plasmids ... Several types of cloning vectors are available ...

http://teacherweb.com/CA/SolanoCommunityCollege/EdwardRe/XXXXX-Biosci-2-Spring-2015-Introduction-to-Molecular-Biology-XXXXX.ppt

Date added: March 29, 2015 - Views: 1

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Ch. 9: Presentation Slides - Tistory

The simplest types of vectors are plasmids whose DNA is double-stranded and circular. When the DNA fragment has been joined to the vector, ... Cloning Vectors: BACs.

http://www.pnumbl.tistory.com/attachment/[email protected]

Date added: June 9, 2015 - Views: 1

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Ligation and Transformation - ISU Plant Genome...

... amplification of gene product A type of cloning vector used to ... of vectors GFP keeps its ... Picture Transformation Plasmids Overall ...

http://www.plantgenomeoutreach.eeob.iastate.edu/teachmod/Bacterial_Transformation.ppt

Date added: October 14, 2011 - Views: 62

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PowerPoint Presentation

DNA ligase splices pieces into plasmids or other cloning vectors How to form recombinant DNA?

http://skywalker.cochise.edu/ashraft/web/BIO101ppt/What%20is%20biotechnology.ppt

Date added: November 8, 2014 - Views: 3

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Biocatalysis - Chatham University

The range of biocatalysis. Oxidation and Reduction. ... These DNAs are called cloning vectors (typically plasmids or viral DNAs). Joining two DNA fragments covalently.

http://moodle.chatham.edu/mod/resource/view.php?id=147629

Date added: March 27, 2015 - Views: 1

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Chapter 15 The Techniques of Molecular Genetics

... (phage and plasmids) ... different cloning vectors must be used in different species. Special cloning vectors can replicate in other prokaryotes and in eukaryotes ...

http://www2.fiu.edu/~barbieri/CH14.ppt

Date added: December 26, 2013 - Views: 14

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DNA Technology & Gene Mapping - Santa Susana High...

DNA Technology & Gene Mapping. ... as they have small circular plasmids that are receptive for insertion of foreign DNA. ... Yeast cells also make good cloning vectors .

http://www.santasusana.org/pakelly/DNA%20Technology%20&%20Gene%20Mapping.pptx

Date added: May 14, 2015 - Views: 1

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No Slide Title

... Restriction enzymes Cloning Vectors Plasmids that can be modified to carry new genes Plasmids useful as cloning vectors must have a replicator (origin of ...

http://faperta.ugm.ac.id/download/pak_tar/genetika-molekuler/cloning20061.ppt

Date added: July 1, 2013 - Views: 2

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No Slide Title

Cloning Vectors and Plasmids Recombinant DNA Transgenic An organism with one or more genes from another organism inserted into its genome Gene Knockout Genes that ...

http://www.lake.k12.fl.us/cms/lib05/FL01000799/Centricity/Domain/2927/Ch_9-4_Genetic_Engineering.ppt

Date added: July 11, 2015 - Views: 1

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PowerPoint Presentation

The cloning process (1 ... conjugation genes oriT: origin of transfer PLASMIDS: APPLICATIONS • Cloning vectors • Gene expression • Transposon delivery ...

http://www.ibt.unam.mx/computo/borra/canovas/dos.ppt

Date added: November 10, 2013 - Views: 21

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Competent Cells formation and transformation of...

Insertion of DNA fragments in to a cloning vector ”e ... Generally plasmid vectors should contain ... There are three general classes for plasmids which can be ...

http://fac.ksu.edu.sa/sites/default/files/1-plasmid_isolation_and_purification.pptx

Date added: October 23, 2014 - Views: 3

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DNA Technology and Genomics

Gene Cloning Method for producing multiple ... as recombinant DNA Bacterial plasmids are commonly used in this process as cloning vectors Cloning a ...

http://mysite.cherokee.k12.ga.us/personal/amanda_dyer/site/Environmental%20Science%20Units/1/DNA%20and%20Protein%20Synthesis/DNA%20Technology%20and%20Genomics.ppt

Date added: March 18, 2015 - Views: 1

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PowerPoint Presentation

Choice of Cloning Vectors: Criteria Small Size: ... Quick recovery of recombinant DNA Examples of Cloning Vectors Bacterial Plasmids: ...

http://public.gettysburg.edu/~hiraizum/bio211s14/0131.ppt

Date added: March 31, 2014 - Views: 1

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DNA Cloning Yields multiple copies of a gene or...

Plasmids are also known as cloning vectors. Cloning Vector – a DNA molecule that can carry foreign DNA into a host cell and replicate there. Examples of Gene Cloning.

http://intranet.oswego308.org/schools/uploads/files/70491/Chapter%2020%20-%20Biotechnology(1).pptx

Date added: May 1, 2015 - Views: 1

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The Genetic Revolution - Mohawk Valley Community...

The Genetic Revolution ... Plasmids a. small circular pieces of DNA found in bacterial cells that carry extrachromosomal ... Cloning A. Notice characteristics of ...

http://www2.mvcc.edu/users/faculty/rthomas/Bi%20102/The%20Genetic%20Revolution.ppt

Date added: January 6, 2013 - Views: 34

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Microbial Genetics - ALRlab

Microbial Genetics WHY? terms Genotype ... steps in cloning Restriction-modification enzymes “shot gun” or PCR Ligase Recombination Plasmids (or phage)= vectors ...

http://alrlab.pdx.edu/media/Lec12.ppt

Date added: May 5, 2013 - Views: 10