Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...
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3 Basic Principles of Recombinant DNA Technology. ... Cosmids. B. Vectors for Other Organisms. ... Engineered Ti plasmids lack the tumor-causing genes, ...
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Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...
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... “sticky” or blunt ends after cleavage Ligation and vectors - plasmids, phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, ...
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Plasmids and other types of cloning vectors were used by Human Genome Project ... Cosmids are artificially constructed cloning vectors that carry up to 45 kb of ...
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Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmids – plasmids containing DNA sequences (cos) ... Molecular Cloning Author: Lab
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Cosmids. yeast artificial chromosome. ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from several base pairs to several ...
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Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...
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Cloning Vectors Enable DNA molecules ... Examples: Cosmids PACs BACs Plasmid Vectors ... Allows you to select bacteria that have plasmids with an ...
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Biotechnology and Genetic Engineering PBIO 4500 ... How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids ...
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Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain ... Cosmids combination of lambda phage and plasmid hold ...
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vectors: Plasmids; Phages . Selectable marker, Restriction sites for cloning. ... Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage. Contain ...
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Ligase: joins (seals) the ends of DNA Vectors: DNA molecule that can replicate & is used to transport foreign DNA Four types of Vectors: Plasmids; Bacteriophage ...
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Viral Vectors May 8 Viral vectors ... plasmid seq cloning site defective virus cosmids HSV genes BACS HSV genes linear plasmid joined end ... gene of interest ...
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Vectors - EM of Small Plasmid DNA Plasmids are vectors, molecular tools for carrying DNA of interest. Other vectors include bacteriophage, cosmids, ...
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Plasmids. Bacteriophages. Cosmids: ... Molecular cloning vectors. Gene cloning. Insulin production by gene cloning. BEST WISHES. Author: Emad A. Morad Created Date:
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Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...
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Why Are Virus’ Used as Cloning Vectors? Genetic engineering produces ... Cosmids are extracted from ... Insulin Cloning Vectors Plasmids Phage BACs and YACs ...
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PLASMIDS PLASMID VECTORS Plasmid vectors are ≈1.2–3kb and contain: ... size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.
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... http://www.whfreeman.com/lodish4e/con_index.htm ... make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids . Title ...
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cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be
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Lambda vectors and their replication ... Cosmids- are hybrid vectors of l phage and plasmids. ... Cosmids- are hybrid vectors of l phage and plasmids.
Date added: September 30, 2013 - Views: 43
... Linear DNA Can insert up to 15 kb fragments Phage Insertion Cosmids Don’t ... Eco R1 Vectors A self-replicating DNA ... of Vectors Plasmids ...
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Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid
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Chapter 4 Molecular Cloning Methods ... Endonucleases Vectors Plasmids as Vectors Phages as Vectors λ Phage Vectors Cosmids M13 phage vectors ...
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Genome Characterization Sequencing, cont’d Physical Mapping
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Section H – Cloning vectors Contents H1 Design of plasmid vectors Ligation products, Twin antibiotic resistance, Blue-white screening, Multiple cloning sites ...
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... F plasmid-based Cosmids: 5 kb plasmids, ... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Specialized cloning vectors expression ...
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Plasmid vectors engineered from bacterial plasmids for use in cloning. ... Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.
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... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids ... Phagemid Vectors Contain components from phage chromosomes and plasmids.
Date added: July 1, 2014 - Views: 6
Trends in Biotechnology 110509 3b – Vectors Chapter 3b - outline V. Cloning Vectors A. Bacterial Vectors 1. Plasmids 2. Bacteriophage 3.
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The original plate is used to pick bacterial cells with recombinant plasmids that hybridized ... Plasmids Phages Cosmids New generation vectors: Bacterial ...
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... insertional vectors and replacement vectors Slide 14 Cosmids are plasmids that can be packaged into bacteriophage lamda particles Slide 16 Slide 17 Slide ...
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Trends in Biotechnology Chapter 3a – Tools for Recombinant DNA
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1 Plasmid vectors 2 Bacteriophage vectors 3 Cosmids 4 BACs & YACs Plasmid ... Plasmid vectors Plasmids are circular DNA molecules present in the cytoplasm of the ...
Date added: December 10, 2013 - Views: 14
Title: Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and Gel Electrophoresis Author: Montarop Yamabhai Created Date: 7/18/2004 7:38:52 AM
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... genetic element used to propagate and express genes of interest in bacteria Plasmids, phages, cosmids, ... color change Plasmids vectors Easy to isolate and ...
Date added: September 7, 2012 - Views: 31
Recombinant DNA Technology for the non-science major. ... Plasmids 2. Bacteriophages 3. Cosmids ... Most vectors are derived from: 1. Plasmids 2.
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Recombinant DNA Technology ... Plasmids for cloning carry drug resistance genes that are used ... plasmids: up to 5 kb phage lambda (λ) vectors (also cosmids): ...
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Why Plasmids are Good Cloning Vectors small size ... Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.
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Recombinant DNA Technology ... Use these phage to infect new E. coli. Cosmids are similar to phage vectors: ... The recombinant plasmids are transformed into E. coli ...
Date added: October 7, 2011 - Views: 262
... when foreign DNA is inserted Recombinant phage DNA can be packaged into viral capsids and used to infect a host cell Cosmids - plasmids ... Plasmids vectors ...
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... Shuttle vectors These are plasmids that can operate in ... inserts up to 40 kbp in size Cosmids in action (fig. 12.9) Shuttle vectors These are plasmids that ...
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One of the most widely used plasmids is pBR322. Phage Vectors Both single- and double-stranded phage ... Cosmids Cosmids are plasmids that contain lambda phage cos ...
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Exploring Genes Recombinant Technololgy
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Lecture 14 – Gene Cloning Based on Chapter 08 - Genomics: The Mapping and Sequencing of Genomes Copyright © 2010 Pearson Education Inc.
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Inquiry into Life Twelfth Edition ... Vectors Plasmids As ... via Plaque Hybridization Cosmids M13 Phage Vectors M13 Cloning to Recover ...
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Prokaryotes don’t glycosylate proteins Plasmids ... sizes when insert included Cloning Vectors 1. Plasmids- 5,000 to 400,000 ... Cosmids- artificially ...
Date added: May 2, 2013 - Views: 47
... specialized plasmids Vectors containing ... coli Plasmids Bacteriophage M13 Lambda Cosmids and ... PowerPoint Presentation Plasmids and ...
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