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3 Basic Principles of Recombinant DNA Technology

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3 Basic Principles of Recombinant DNA Technology

3 Basic Principles of Recombinant DNA Technology. ... Cosmids. B. Vectors for Other Organisms. ... Engineered Ti plasmids lack the tumor-causing genes, ...

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: May 26, 2012 - Views: 182

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Cloning - Sam Houston State University

Plasmids and other types of cloning vectors were used by Human Genome Project ... Cosmids are artificially constructed cloning vectors that carry up to 45 kb of ...

http://www.shsu.edu/~agr_www/documents/CloningPart1PP.pptx

Date added: August 27, 2013 - Views: 49

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Cloning into Plasmids - Buffalo State College

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 104

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No Slide Title

Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: May 6, 2013 - Views: 16

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Plasmid DNA isolation - Assiut University

Cosmids. yeast artificial chromosome. ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from several base pairs to several ...

http://www.aun.edu.eg/molecular_biology/Procedure%20from%20gene%20to%20protein/2%20Cloning%20vectors.pptx

Date added: January 25, 2015 - Views: 11

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No Slide Title

... “sticky” or blunt ends after cleavage Ligation and vectors - plasmids, phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, ...

http://web.bio.utk.edu/humangenetics/lecture/molec_tools06sep02.ppt

Date added: November 28, 2013 - Views: 4

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PowerPoint Presentation

Lambda Bacteriophage Vectors cos cos cos Selection: Only recombinant phage produce plaques. 1. Size ... Cosmids: Plasmids that contain l cos sites.

http://faculty.smu.edu/borr/ClassNotes/10-17-05.ppt

Date added: January 4, 2015 - Views: 1

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PowerPoint Presentation

Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...

http://faculty.smu.edu/borr/ClassNotes/10-19-05.ppt

Date added: August 2, 2013 - Views: 6

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No Slide Title

Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain ... Cosmids combination of lambda phage and plasmid hold ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: March 14, 2012 - Views: 90

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Biotechnology and Genetic Engineering-PBIO 450/550

DNA cloning & cloning vectors ... gene libraries How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids . Title ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20427%20&%20527/PBIO427%20Lecture%202%202009.ppt

Date added: May 22, 2013 - Views: 16

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Biotechnology and Genetic Engineering-PBIO 450/550

... but it can also be 125I Animations for two related uses of expression vectors ... vectors including plasmids, bacteriophage lambda (l), and cosmids ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB7200%20Lecture%203.ppt

Date added: February 13, 2013 - Views: 52

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Inquiry into Life Twelfth Edition - Regis...

vectors: Plasmids; Phages . ... Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage. ... Inquiry into Life Twelfth Edition

http://academic.regis.edu/jbetz/BL426Lectures-Notes/chapt04%202011.pps

Date added: August 2, 2013 - Views: 49

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Viral Vectors - homepage.usask.ca

Viral Vectors May 8 Viral vectors ... plasmid seq cloning site defective virus cosmids HSV genes BACS HSV genes linear plasmid joined end ... gene of interest ...

http://homepage.usask.ca/~vim458/vtmc831/May8.ppt

Date added: February 19, 2012 - Views: 48

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PowerPoint Presentation

Vectors - EM of Small Plasmid DNA Plasmids are vectors, molecular tools for carrying DNA of interest. Other vectors include bacteriophage, cosmids, ...

http://people.cst.cmich.edu/schis1j/bio326-02/lectures/11-8.ppt

Date added: January 13, 2013 - Views: 22

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Plasmids and Vectors - Molecular Biology for...

Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...

https://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: January 31, 2015 - Views: 8

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PowerPoint Presentation

Molecular Basis for Relationship between Genotype and Phenotype DNA genotype DNA sequence transcription RNA translation amino acid sequence protein function

http://public.gettysburg.edu/~hiraizum/bio211/0909.ppt

Date added: September 8, 2014 - Views: 2

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Slide 1

Key concepts of Molecular Cloning. What is gene/ molecular cloning? How does it differ from cloning an entire organism? Molecular cloning. refers to the procedure of ...

http://scripts.biosummerschool.com/archive/2013/28.5.92.Summerschool%20slides.%20cloning.pptx

Date added: February 14, 2014 - Views: 6

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Competent Cells formation and transformation of...

Three features of all cloning vectors ... Cosmids: an extra chromosomal circular DNA molecule that combines features of plasmids and phage; cloning limit ...

http://fac.ksu.edu.sa/sites/default/files/2-competent_cells_formation_and_transformation_of_competent_cells_with_dna_0.pptx

Date added: March 27, 2015 - Views: 1

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Foundations in Microbiology - learning.hccs.edu

Chapter 10 Genetic Engineering: A Revolution in Molecular Biology

http://learning.hccs.edu/faculty/idowu.famuyiwa/biol2420/lecture-notes/chapter-10

Date added: April 24, 2015 - Views: 6

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Vectors - College of Computer, Mathematical, and...

Cosmids are extracted from ... rDNA Objectives Steps in rDNA Processing Genetic Engineering Advances The use of rDNA to Produce Human Insulin Cloning Vectors Plasmids ...

http://www.clfs.umd.edu/grad/mlfsc/res/RecombomamtDNA&Medicine.ppt

Date added: September 11, 2011 - Views: 282

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PowerPoint Presentation

cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be

http://genemol.org/molecular-bio/7-Artificial-chromosomes/YAC-BAC-PAC.ppt

Date added: October 7, 2011 - Views: 229

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Chapter 12

... General PowerPoint Presentation Types of Vectors and DNA delivery systems Types of Vectors Plasmids Plasmid vectors ... vectors Phage Insertion Cosmids ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: May 3, 2013 - Views: 37

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Lambda Vectors and their replication - Biology

Lambda vectors and their replication Sonita Gafary Biochem 72020 Lambda was first discovered at the Pasteur Institute by Andre Lwoff when he observed strains of E. Coli.

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: September 30, 2013 - Views: 49

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Slide 1

Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid

http://ecourse.sl.on.ca/mjsibbald/mjsibbald/08mb/0807mbviral%20vectors%20and%20other%20vectors.ppt

Date added: May 2, 2013 - Views: 25

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DNA cloning intro.ppt - Biology

PLASMIDS PLASMID VECTORS Plasmid vectors are ≈1.2–3kb and contain: ... size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202012%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: January 23, 2013 - Views: 35

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Chapter 4 Molecular Cloning Methods - Xiamen...

Chapter 4 Molecular Cloning Methods ... Endonucleases Vectors Plasmids as Vectors Phages as Vectors λ Phage Vectors Cosmids M13 phage vectors ...

http://yjsy.xmu.edu.cn/Cn/uploads/Chapter%204.ppt

Date added: January 28, 2012 - Views: 182

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Vectors for Even Larger Genomic DNA Inserts

General Microbiology (Micr300) Lecture 11 Biotechnology (Text Chapters: 10.15-10.17; 31.1-31.10)

http://instructional1.calstatela.edu/hxu3/Micr300/S06micr300Lec11final.ppt

Date added: May 29, 2013 - Views: 26

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Genetic Engineering - University of North Carolina...

Genetic Engineering ... Vector Stable Packaging Cosmids Other Vectors Expression Vectors Secretion ... Splice into Cloning Vehicle Plasmids Viruses Use ...

p://people.uncw.edu/sizemorer

Date added: November 5, 2014 - Views: 202

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Setting up a transformation--how will the...

... F plasmid-based Cosmids: 5 kb plasmids, ... vectors and tags vectors for large pieces of DNA, e.g. Cosmids and ... scheme PowerPoint Presentation ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week5.ppt

Date added: November 15, 2011 - Views: 84

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Section D - Prokaryotic and Eukaryotic Chromosome...

H4 Eukaryotic vectors — Yeast episomal plasmids Vectors for the cloning and expression of genes ... Hybrid plasmid-M13 vectors H3 Cosmids, ...

http://fzswx.gxu.edu.cn/course/Section%20H%20-%20Cloning%20vectors.ppt

Date added: February 13, 2012 - Views: 25

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Trends in Biotechnology - hallym.ac.kr

Trends in Biotechnology 110509 3b – Vectors ...

http://web.hallym.ac.kr/~colinbrowne/TrendsBiotechnology_files/110509%203b.ppt

Date added: December 5, 2013 - Views: 14

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A DNS tulajdonságai hogyan használhatók a...

A DNS tulajdonságai hogyan használhatók a manipulációban? Könnyen izolálható, elektroforézis, Összeilleszthető Megolvasztható, kelálható festékekkel ...

http://biokemia.elte.hu/attachments/download/course/33/23

Date added: January 30, 2016 - Views: 1

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Trends in Biotechnology - web.hallym.ac.kr

Trends in Biotechnology Chapter 3a – Tools for Recombinant DNA

http://web.hallym.ac.kr/~colinbrowne/TrendsBiotechnology_files/110502%203a%20-%20Tools%20for%20Recombinant%20DNA.ppt

Date added: December 9, 2013 - Views: 3

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PowerPoint Presentation

Plasmid vectors engineered from bacterial plasmids for use in cloning. ... Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.

http://www.bio.miami.edu/mccracken/genetics/lectures/chapter_08.ppt

Date added: April 10, 2015 - Views: 1

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Molecular Cloning I: Restriction Enzymes, PCR, ...

Title: Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and Gel Electrophoresis Author: Montarop Yamabhai Created Date: 7/18/2004 7:38:52 AM

http://www.sut.ac.th/iat/biotech/MontaropSite/Powerpoint/304512_2551.ppt

Date added: October 7, 2011 - Views: 329

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Part II: Recombinant DNA Technology.ppt - Rajesh...

Title: Part II: Recombinant DNA Technology.ppt Author: ITR Last modified by: Konkuk university Created Date: 1/20/2002 6:56:56 PM Document presentation format

http://www.rajeshbanu.com/ebt/molecular%20breeding_RDT_2.ppt

Date added: March 1, 2016 - Views: 1

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Recombinant DNA Technology for the non-science...

Recombinant DNA Technology for the non-science major. ... Plasmids 2. Bacteriophages 3. Cosmids ... Most vectors are derived from: 1. Plasmids 2.

http://cogs.csustan.edu/~tom/bioinfo/ppt/Day3.ppt

Date added: October 16, 2012 - Views: 32

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Enzyme Mechanisms - Illinois Institute of...

... Middle third of its 48.5-kbp chromosome isn’t needed for infection Cosmids 14 ... Shuttle vectors These are plasmids that can ... Enzyme Mechanisms ...

http://csrri.iit.edu/~howard/biochem/lecppt/mobio_methods.ppt

Date added: May 12, 2013 - Views: 13

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Freeman 1e: How we got there - oakland.edu

Plasmids as Cloning Vectors Plasmids are useful ... Plasmid vectors containing the lambda cos sites are called cosmids, ... Freeman 1e: How we got there Author:

http://www2.oakland.edu/biology/chaudhry/pics/10.BacterialGenetics.ppt

Date added: May 7, 2012 - Views: 20

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Slide 1

The original plate is used to pick bacterial cells with recombinant plasmids that hybridized ... Plasmids Phages Cosmids New generation vectors: Bacterial ...

http://higheredbcs.wiley.com/legacy/college/allison/1118059816/ppt/ch08.ppt

Date added: August 2, 2013 - Views: 25

ppt
PowerPoint Presentation

... specialized plasmids Vectors containing ... coli Plasmids Bacteriophage M13 Lambda Cosmids and ... PowerPoint Presentation Plasmids and ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week4.ppt

Date added: October 3, 2011 - Views: 111

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DNA Libraries - Western Washington University

... plasmids BACS YACS Transduction (via virus mechanisms); ... (via virus mechanisms); phage cosmids Cloning Step 3 ...vectors contain selectable markers, ...

http://fire.biol.wwu.edu/young/321/LG8.ppt

Date added: September 20, 2011 - Views: 33

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Slide 1

... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids ... Vectors Slide 11 Plasmid Vectors Plasmids Slide 14 ...

http://getenotes.weebly.com/uploads/1/4/1/9/14194145/genetic_engineering__unit_i__lecture_1.ppt

Date added: August 23, 2014 - Views: 6

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Inquiry into Life Twelfth Edition - McGraw Hill...

... Phagemids are also vectors Like cosmids have aspects of ... classes of vectors: Plasmids Phages Plasmids ... Inquiry into Life Twelfth Edition Author:

http://highered.mheducation.com/sites/dl/free/0072995246/444682/chapt04_lecture.ppt

Date added: July 14, 2014 - Views: 42

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Slide 1

... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids phasmids Phagemids BAC ... Yeast episomal plasmids YEps were ...

http://getenotes.weebly.com/uploads/1/4/1/9/14194145/genetic_engineering__unit_i__lecture_4.ppt

Date added: June 18, 2014 - Views: 11

ppt
Slide 1

Lecture 14 – Gene Cloning Based on Chapter 08 - Genomics: The Mapping and Sequencing of Genomes Copyright © 2010 Pearson Education Inc.

http://www.auburn.edu/academic/classes/biol/3020/lecture_ppts/L14_DNA%20Cloning_14F.ppt

Date added: November 5, 2014 - Views: 2

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Slajd 1 - Strona Główna - Politechnika Gdańska

Essential Features of Plasmids Most vectors currently in use carry a replicon from pMB1 or Col ... Cosmids M13 Phage Vectors Copyright © The McGraw-Hill Companies ...

http://www.pg.gda.pl/chem/Katedry/Mikrobiologia/W2-IG2.ppt

Date added: November 21, 2011 - Views: 38

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Slide 1

Why Plasmids are Good Cloning Vectors small size ... Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.

http://mb206.wikispaces.com/file/view/MB206_fhs_int_008b_AT_Jan09.ppt

Date added: June 1, 2013 - Views: 9

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PowerPoint Presentation

Cloning Vectors Target Gene ... structure Construct expression plasmids for functional studies of Protein X in cells Mutagenize ... Bacteriophage-based Cosmids: ...

http://users.umassmed.edu/maria.zapp/Block2Lecture1%2011_01_04.ppt

Date added: October 7, 2011 - Views: 122