Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...
Date added: December 2, 2011 - Views: 95
3 Basic Principles of Recombinant DNA Technology. ... Cosmids. B. Vectors for Other Organisms. ... Engineered Ti plasmids lack the tumor-causing genes, ...
Date added: May 26, 2012 - Views: 173
Vectors (cloning vehicles) Enzymes for cutting and joining the DNA fragments. ... Types of Vectors. Plasmids. Cosmids. Fosmids. Phages. Yeast Artificial Chromosomes ...
Date added: October 7, 2015 - Views: 1
Lambda Bacteriophage Vectors cos cos cos Selection: Only recombinant phage produce plaques. 1. Size ... Cosmids: Plasmids that contain l cos sites.
Date added: January 4, 2015 - Views: 1
Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...
Date added: May 6, 2013 - Views: 7
... “sticky” or blunt ends after cleavage Ligation and vectors - plasmids, phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, ...
Date added: November 28, 2013 - Views: 4
Cosmids. yeast artificial chromosome. ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from several base pairs to several ...
Date added: January 25, 2015 - Views: 3
Plasmids and other types of cloning vectors were used by Human Genome Project ... Cosmids are artificially constructed cloning vectors that carry up to 45 kb of ...
Date added: August 27, 2013 - Views: 39
Cloning Vectors Enable DNA molecules ... Examples: Cosmids PACs BACs Plasmid Vectors ... Allows you to select bacteria that have plasmids with an ...
Date added: May 13, 2013 - Views: 27
Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain ... Cosmids combination of lambda phage and plasmid hold ...
Date added: March 14, 2012 - Views: 78
vectors: Plasmids; Phages . Selectable marker, Restriction sites for cloning. ... Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage. Contain ...
Date added: August 2, 2013 - Views: 41
... but it can also be 125I Animations for two related uses of expression vectors Expression ... cloning vectors including plasmids, ... cDNA library construction ...
Date added: February 13, 2013 - Views: 46
Viral Vectors May 8 Viral vectors ... plasmid seq cloning site defective virus cosmids HSV genes BACS HSV genes linear plasmid joined end ... gene of interest ...
Date added: February 19, 2012 - Views: 42
Biotechnology and Genetic Engineering PBIO 450 ... How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids . Title ...
Date added: October 6, 2011 - Views: 28
Ligase: joins (seals) the ends of DNA Vectors: DNA molecule that can replicate & is used to transport foreign DNA Four types of Vectors: Plasmids; Bacteriophage ...
Date added: August 2, 2013 - Views: 15
Vectors - EM of Small Plasmid DNA Plasmids are vectors, molecular tools for carrying DNA of interest. Other vectors include bacteriophage, cosmids, ...
Date added: January 13, 2013 - Views: 22
Molecular Basis for Relationship between Genotype and Phenotype DNA genotype DNA sequence transcription RNA translation amino acid sequence protein function
Date added: September 8, 2014 - Views: 2
Vectors. Plasmids, Lambda ... fewer clones required than for cosmids ... These genomic fragments are then either cloned into vectors & introduced into a microbe or ...
Date added: June 3, 2014 - Views: 2
Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...
Date added: January 31, 2015 - Views: 7
Plasmids. Bacteriophages. Cosmids: ... Molecular cloning vectors. Gene cloning. Insulin production by gene cloning. BEST WISHES. Author: Emad A. Morad Created Date:
Date added: December 16, 2014 - Views: 2
Chapter 10 Genetic Engineering: A Revolution in Molecular Biology
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Why Are Virus’ Used as Cloning Vectors? Genetic engineering produces ... Cosmids are extracted from ... Insulin Cloning Vectors Plasmids Phage BACs and YACs ...
Date added: September 11, 2011 - Views: 270
cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be
Date added: October 7, 2011 - Views: 229
PLASMIDS PLASMID VECTORS Plasmid vectors are ≈1.2–3kb and contain: ... size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.
Date added: January 23, 2013 - Views: 35
... Linear DNA Can insert up to 15 kb fragments Phage Insertion Cosmids Don’t ... Eco R1 Vectors A self-replicating DNA ... of Vectors Plasmids ...
Date added: May 3, 2013 - Views: 35
Lambda vectors and their replication ... Cosmids- are hybrid vectors of l phage and plasmids. ... Cosmids- are hybrid vectors of l phage and plasmids.
Date added: September 30, 2013 - Views: 46
Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid
Date added: May 2, 2013 - Views: 21
Chapter 4 Molecular Cloning Methods ... Endonucleases Vectors Plasmids as Vectors Phages as Vectors λ Phage Vectors Cosmids M13 phage vectors ...
Date added: January 28, 2012 - Views: 177
Section H – Cloning vectors Contents H1 Design of plasmid vectors Ligation products, Twin antibiotic resistance, Blue-white screening, Multiple cloning sites ...
Date added: February 13, 2012 - Views: 15
... F plasmid-based Cosmids: 5 kb plasmids, ... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Specialized cloning vectors expression ...
Date added: November 15, 2011 - Views: 78
Isolate Gene Fragmented Total DNA Use mRNA and Make DNA with Reverse Transcriptase Splice into Cloning Vehicle Plasmids ... Cosmids Other Vectors ...
Date added: November 5, 2014 - Views: 131
Trends in Biotechnology 110509 3b – Vectors
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... insertional vectors and replacement vectors Slide 14 Cosmids are plasmids that can be packaged into bacteriophage lamda particles Slide 16 Slide 17 Slide ...
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Plasmid vectors engineered from bacterial plasmids for use in cloning. ... Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.
Date added: October 2, 2014 - Views: 4
... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids ... Phagemid Vectors Contain components from phage chromosomes and plasmids.
Date added: July 1, 2014 - Views: 6
Trends in Biotechnology Chapter 3a – Tools for Recombinant DNA
Date added: December 9, 2013 - Views: 3
Why Plasmids are Good Cloning Vectors small size ... Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.
Date added: June 1, 2013 - Views: 9
1 Plasmid vectors 2 Bacteriophage vectors 3 Cosmids 4 BACs & YACs Plasmid ... Plasmid vectors Plasmids are circular DNA molecules present in the cytoplasm of the ...
Date added: December 10, 2013 - Views: 15
The original plate is used to pick bacterial cells with recombinant plasmids that hybridized ... Plasmids Phages Cosmids New generation vectors: Bacterial ...
Date added: August 2, 2013 - Views: 24
Title: Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and Gel Electrophoresis Author: Montarop Yamabhai Created Date: 7/18/2004 7:38:52 AM
Date added: October 7, 2011 - Views: 307
Recombinant DNA Technology for the non-science major. ... Plasmids 2. Bacteriophages 3. Cosmids ... Most vectors are derived from: 1. Plasmids 2.
Date added: October 16, 2012 - Views: 32
Slide 8 - Cloning Vectors - Plasmid ... Cosmids have an origin ... so blue-white screening can indicate which colonies contain recombinant plasmids. Slide 9 ...
Date added: November 5, 2014 - Views: 2
Recombinant DNA Technology ... Plasmids for cloning carry drug resistance genes that are used ... plasmids: up to 5 kb phage lambda (λ) vectors (also cosmids): ...
Date added: May 8, 2012 - Views: 59
... when foreign DNA is inserted Recombinant phage DNA can be packaged into viral capsids and used to infect a host cell Cosmids - plasmids ... Plasmids vectors ...
Date added: September 15, 2012 - Views: 20
Recombinant DNA Technology ... Use these phage to infect new E. coli. Cosmids are similar to phage vectors: ... The recombinant plasmids are transformed into E. coli ...
Date added: October 7, 2011 - Views: 265
... Shuttle vectors These are plasmids that can operate in ... inserts up to 40 kbp in size Cosmids in action (fig. 12.9) Shuttle vectors These are plasmids that ...
Date added: May 12, 2013 - Views: 9
Exploring Genes Recombinant Technololgy
Date added: May 17, 2013 - Views: 28
One of the most widely used plasmids is pBR322. Phage Vectors Both single- and double-stranded phage ... Cosmids Cosmids are plasmids that contain lambda phage cos ...
Date added: November 2, 2013 - Views: 3