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Cloning into Plasmids - Buffalo State College

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Cloning into Plasmids - Buffalo State College

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 95

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3 Basic Principles of Recombinant DNA Technology

3 Basic Principles of Recombinant DNA Technology. ... A. Bacterial Vectors. 1. Plasmids. ... Cosmids. B. Vectors for Other Organisms. 1. Yeast Artificial Chromosomes ...

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: May 26, 2012 - Views: 169

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PowerPoint Presentation

cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be

http://genemol.org/molecular-bio/7-Artificial-chromosomes/YAC-BAC-PAC.ppt

Date added: October 7, 2011 - Views: 227

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Cloning - Sam Houston State University

Plasmids and other types of cloning vectors were used by Human Genome Project ... Cosmids are artificially constructed cloning vectors that carry up to 45 kb of ...

http://www.shsu.edu/~agr_www/documents/CloningPart1PP.pptx

Date added: August 27, 2013 - Views: 29

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No Slide Title

Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: May 6, 2013 - Views: 4

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Slide 1

Cosmids. yeast artificial chromosome. ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from several base pairs to several ...

http://www.aun.edu.eg/molecular_biology/Procedure%20from%20gene%20to%20protein/2%20Cloning%20vectors.pptx

Date added: January 25, 2015 - Views: 1

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PowerPoint Presentation

Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...

http://faculty.smu.edu/borr/ClassNotes/10-19-05.ppt

Date added: August 2, 2013 - Views: 4

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No Slide Title

... “sticky” or blunt ends after cleavage Ligation and vectors - plasmids, phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, ...

http://web.bio.utk.edu/humangenetics/lecture/molec_tools06sep02.ppt

Date added: November 28, 2013 - Views: 4

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PowerPoint Presentation

Cloning Vectors Enable DNA molecules ... Examples: Cosmids PACs BACs Plasmid Vectors ... Allows you to select bacteria that have plasmids with an ...

http://faculty.smu.edu/borr/ClassNotes/10-14-05.ppt

Date added: May 13, 2013 - Views: 26

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Biotechnology and Genetic Engineering-PBIO 450/550

Biotechnology and Genetic Engineering PBIO 450 ... How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids . Title ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO450Lecture308.ppt

Date added: October 6, 2011 - Views: 28

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Introduction to molecular cloning - ABE

Plasmids – used in cloning ... Cosmidsplasmids ... small circular dsDNA that autonomously replicates apart from the chromosome of the host cell “molecular ...

http://abe.leeward.hawaii.edu/Summer07/Introduction%20to%20Molecular%20Cloning.ppt

Date added: November 5, 2011 - Views: 89

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Inquiry into Life Twelfth Edition - Regis...

vectors: Plasmids; Phages . Selectable marker, Restriction sites for cloning. ... Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage. Contain ...

http://academic.regis.edu/jbetz/BL426Lectures-Notes/chapt04%202011.pps

Date added: August 2, 2013 - Views: 38

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No Slide Title

Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain ... Cosmids combination of lambda phage and plasmid hold ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: March 14, 2012 - Views: 78

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PowerPoint Presentation

Ligase: joins (seals) the ends of DNA Vectors: DNA molecule that can replicate & is used to transport foreign DNA Four types of Vectors: Plasmids; Bacteriophage ...

http://www.oucom.ohiou.edu/dbms-james/Winter_2003/Recomb%20DNA%20Tech.ppt

Date added: August 2, 2013 - Views: 14

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Viral Vectors - University of Saskatchewan

Viral Vectors May 8 Viral vectors ... plasmid seq cloning site defective virus cosmids HSV genes BACS HSV genes linear plasmid joined end ... gene of interest ...

http://homepage.usask.ca/~vim458/vtmc831/May8.ppt

Date added: February 19, 2012 - Views: 39

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PowerPoint Presentation

Vectors - EM of Small Plasmid DNA Plasmids are vectors, molecular tools for carrying DNA of interest. Other vectors include bacteriophage, cosmids, ...

http://people.cst.cmich.edu/schis1j/bio326-02/lectures/11-8.ppt

Date added: January 13, 2013 - Views: 21

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الشريحة 1 - Majmaah University

Plasmids. Bacteriophages. Cosmids: ... Molecular cloning vectors. Gene cloning. Insulin production by gene cloning. BEST WISHES. Author: Emad A. Morad Created Date:

http://faculty.mu.edu.sa/public/uploads/1385287329.674610.%20gene%20cloning.pptx

Date added: December 16, 2014 - Views: 2

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PowerPoint Presentation

Molecular Basis for Relationship between Genotype and Phenotype DNA genotype DNA sequence transcription RNA translation amino acid sequence protein function

http://public.gettysburg.edu/~hiraizum/bio211/0909.ppt

Date added: September 8, 2014 - Views: 1

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基因重組原理與技術

... (Cloning Vectors) 細菌 – 質體 (plasmids) 噬菌體 (bacteriophages) 黏接質體 ... (Cloning Vectors) 噬菌體與 cosmids 舉例 轉殖載體 ...

http://elearning.ice.ntnu.edu.tw/km/Data/Teacher/31972/data/%E6%88%91%E7%9A%84%E5%80%8B%E4%BA%BA%E6%96%87%E4%BB%B6/lecture%202%20-%20%E5%9F%BA%E5%9B%A0%E9%87%8D%E7%B5%84%E5%8E%9F%E7%90%86%E8%88%87%E6%8A%80%E8%A1%93-eef23.ppt

Date added: March 29, 2012 - Views: 2

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Biotechnology and Genetic Engineering-PBIO 450/550

... but it can also be 125I Animations for two related uses of expression vectors Expression ... cloning vectors including plasmids, ... cDNA library construction ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB7200%20Lecture%203.ppt

Date added: February 13, 2013 - Views: 41

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Vectors - College of Computer, Mathematical, and...

Cosmids combine essential elements of a plasmid and Lambda systems. Cosmids are extracted from bacteria and mixed with ... Plasmid Cloning Vectors Plasmids are ...

http://www.clfs.umd.edu/grad/mlfsc/res/RecombomamtDNA&Medicine.ppt

Date added: September 11, 2011 - Views: 267

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Section D - Prokaryotic and Eukaryotic Chromosome...

Section G – Gene manipulation Contents G1 DNA cloning: an overview DNA cloning, Hosts and vectors, Subcloning, DNA libraries, Screening libraries, Analysis of a ...

http://fzswx.gxu.edu.cn/course/Section%20G%20-%20Gene%20manipulation.ppt

Date added: November 27, 2013 - Views: 11

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Plasmids and Vectors - Molecular Biology for...

Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...

https://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: January 31, 2015 - Views: 2

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PowerPoint 演示文稿

... Plasmid vecters 2 Bacteriophage vectors 3 Cosmids & BACs 4 Eukaryotic vectors ... libraries from prokaryotes in plasmids where the insert size is 5-10kb ...

http://202.114.65.51/fzjx/fzswx/wlkt/ppt2005/application1.ppt

Date added: March 2, 2012 - Views: 9

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Chpter 4

Chapter 4 Molecular Cloning Methods Gene Cloning The Role of Restriction Endonuclease Vectors Plasmids : carriers ... Cosmids * cohesive ends of l phage DNA ...

http://memo.cgu.edu.tw/B-CB/old_v2/bstl/slide/G/Molecular%20%20Biology%20%20Chpter%204.ppt

Date added: February 21, 2013 - Views: 14

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幻灯片 1

The four major types of vectors are plasmids (质粒DNA) car bacteriophages (噬菌体DNA) minivan viruses, cosmids (病毒, 柯斯质粒) truck ...

http://biolab.szu.edu.cn/otherweb/lzc/genetic%20engineering/courseware/g3.ppt

Date added: June 18, 2013 - Views: 3

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Lambda Vectors and their replication

Lambda vectors and their ... Plasmids- small circular DNA molecules which can replicate their DNA independently ... Cosmids- are hybrid vectors of l phage and ...

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: September 30, 2013 - Views: 41

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Slide 1

Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid

http://ecourse.sl.on.ca/mjsibbald/mjsibbald/08mb/0807mbviral%20vectors%20and%20other%20vectors.ppt

Date added: May 2, 2013 - Views: 20

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PowerPoint Presentation

Gene expression * Expression vectors: ... E. coli cloning vector (circular): plasmids (质粒 ... plasmid-bacteriophage l hybrids (cosmids) (考斯质粒 ...

http://202.114.65.51/fzjx/fzswx/wlkt/ppt2008/2008chapter21.ppt

Date added: November 11, 2011 - Views: 13

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第六章 - 同济大学医学院

Vectors: plasmid, λphage, ... Plasmids usually occur naturally in bacteria, ... Cosmids can be used to build genomic library.

http://med.tongji.edu.cn/yzkc/DT_UploadFile/20101224183754546.ppt

Date added: June 13, 2012 - Views: 21

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投影片 1

... Insertional inactivation Antibiotic resistance gene B/W selection Blue white screening SPECIFIC TYPES OF CLONING VECTORS ... Cosmids More deletion of ... plasmids ...

http://lms.ls.ntou.edu.tw/sys/read_attach.php?id=10604

Date added: June 26, 2015 - Views: 1

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Chapter 12

... Linear DNA Can insert up to 15 kb fragments Phage Insertion Cosmids Don’t ... Eco R1 Vectors A self-replicating DNA ... of Vectors Plasmids ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: May 3, 2013 - Views: 33

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Chapter 4 Molecular Cloning Methods - Xiamen...

Chapter 4 Molecular Cloning Methods ... Endonucleases Vectors Plasmids as Vectors Phages as Vectors λ Phage Vectors Cosmids M13 phage vectors ...

http://yjsy.xmu.edu.cn/Cn/uploads/Chapter%204.ppt

Date added: January 28, 2012 - Views: 173

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DNA cloning intro.ppt - Biology

PLASMIDS PLASMID VECTORS Plasmid vectors are ≈1.2–3kb and contain: ... size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202012%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: January 23, 2013 - Views: 34

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Chpter 4

Chapter 4 Molecular Cloning Methods

http://memo.cgu.edu.tw/tzu-chien/MB2009/Yu2009-1.ppt

Date added: May 10, 2013 - Views: 20

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Section D - Prokaryotic and Eukaryotic Chromosome...

H4 Eukaryotic vectors — Yeast episomal plasmids Vectors for the cloning and expression of genes ... Hybrid plasmid-M13 vectors H3 Cosmids, ...

http://fzswx.gxu.edu.cn/course/Section%20H%20-%20Cloning%20vectors.ppt

Date added: February 13, 2012 - Views: 15

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Slide 1

... genetic element used to propagate and express genes of interest in bacteria Plasmids, phages, cosmids, ... color change Plasmids vectors Easy to isolate and ...

http://classes.biology.ucsd.edu/bimm120.WI07/genetics2_doran_fa06.ppt

Date added: September 7, 2012 - Views: 31

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Setting up a transformation--how will the...

... F plasmid-based Cosmids: 5 kb plasmids, ... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Specialized cloning vectors expression ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week5.ppt

Date added: November 15, 2011 - Views: 77

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Protein Structure

Genome Characterization Sequencing, cont’d Physical Mapping

http://www.uky.edu/Classes/BIO/520/BIO520WWW/Lecture2/lect20/lect20.ppt

Date added: November 12, 2014 - Views: 1

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PowerPoint Presentation

Plasmid vectors engineered from bacterial plasmids for use in cloning. ... Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.

http://www.bio.miami.edu/mccracken/genetics/lectures/chapter_08.ppt

Date added: April 10, 2015 - Views: 1

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Slide 1

... when foreign DNA is inserted Recombinant phage DNA can be packaged into viral capsids and used to infect a host cell Cosmids - plasmids ... Plasmids vectors ...

http://classes.biology.ucsd.edu/old.web.classes/bimm120.WI06/john_buchanan_lec2.ppt

Date added: September 15, 2012 - Views: 20

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Trends in Biotechnology - hallym.ac.kr

Trends in Biotechnology 110509 3b – Vectors Chapter 3b - outline V. Cloning Vectors A. Bacterial Vectors 1. Plasmids 2. Bacteriophage 3.

http://web.hallym.ac.kr/~colinbrowne/TrendsBiotechnology_files/110509%203b.ppt

Date added: December 5, 2013 - Views: 10

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Recombinant DNA Technology

Recombinant DNA Technology ... Plasmids for cloning carry drug resistance genes that are used ... plasmids: up to 5 kb phage lambda (λ) vectors (also cosmids): ...

http://www.bios.niu.edu/johns/bioinf_intro/Recombinant%20DNA%20Technology.ppt

Date added: May 8, 2012 - Views: 58

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Slide 1

... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids ... Phagemid Vectors Contain components from phage chromosomes and plasmids.

http://getenotes.weebly.com/uploads/1/4/1/9/14194145/genetic_engineering__unit_i__lecture_3.ppt

Date added: July 1, 2014 - Views: 6

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Recombinant DNA Technology - NIU Department of...

Recombinant DNA Technology ... Plasmids for cloning carry drug resistance genes ... Use these phage to infect new E. coli. Cosmids are similar to phage vectors: ...

http://www.bios.niu.edu/johns/humgen/Recombinant_DNA_Technology.ppt

Date added: October 7, 2011 - Views: 262

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Slide 1

... insertional vectors and replacement vectors Slide 14 Cosmids are plasmids that can be packaged into bacteriophage lamda particles Slide 16 Slide 17 Slide ...

http://getenotes.weebly.com/uploads/1/4/1/9/14194145/genetic_engineering__unit_i__lecture_2.ppt

Date added: August 20, 2014 - Views: 1

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Molecular Cloning I: Restriction Enzymes, PCR, ...

Title: Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and Gel Electrophoresis Author: Montarop Yamabhai Created Date: 7/18/2004 7:38:52 AM

http://www.sut.ac.th/iat/biotech/MontaropSite/Powerpoint/304512_2551.ppt

Date added: October 7, 2011 - Views: 302

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Slide 1

The original plate is used to pick bacterial cells with recombinant plasmids that hybridized ... Plasmids Phages Cosmids New generation vectors: Bacterial ...

http://higheredbcs.wiley.com/legacy/college/allison/1118059816/ppt/ch08.ppt

Date added: August 2, 2013 - Views: 24

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Trends in Biotechnology - hallym.ac.kr

Trends in Biotechnology ... 3. Cosmids B. Vectors for Other ... DNA DNA Cloning V. Cloning Vectors A. Bacterial Vectors 1. Plasmids 2.

http://web.hallym.ac.kr/~colinbrowne/TrendsBiotechnology_files/110502%203a%20-%20Tools%20for%20Recombinant%20DNA.ppt

Date added: December 9, 2013 - Views: 3