Chapter 3-Contd. Western blotting & SDS-PAGE Western Blot Western blots allow investigators to determine the molecular weight of a protein and to measure relative ...
Date added: November 1, 2011 - Views: 264
Southern Blotting A method ... segment Wash away unbound probe Detect probes using x-ray film autoradiograph RFLP Animations Animation #1 Animation #2 ...
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... Southern blotting DNA sequencing Human genome project Polymerase chain reaction (PCR) ... very specific link to animation Restriction site: ...
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Fluorescence in situ Hybridization ... genome than other conventional methods like Southern blotting FISH Procedure Denature the chromosomes Denature the ...
Date added: February 17, 2013 - Views: 211
PCR Animation DNA Fingerprinting Used to identify individuals by their repeat regions (usually STR) regions: Steps involved: ... (Southern blotting) ...
Date added: August 4, 2015 - Views: 2
PBIO 427/527: Molecular Genetics Lecture 3 - Review Southern, Northern & Western blotting DNA sequencing Polymerase chain reaction (PCR) DNA microarrays
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Student Learning Outcomes. Define taxonomy, taxon, and phylogeny. List the characteristics of the Bacteria, Archaea, and Eukarya domains. Differentiate among ...
Date added: May 23, 2015 - Views: 6
Hybridization DNA hybridization Southern blotting ... Primer extension Fig. 3.8 The Polymerase Chain Reaction Go to Animation courtesy of the following websites: ...
Date added: July 1, 2012 - Views: 32
DNA Cloning and Its Applications: A Preview. Most methods for cloning pieces of DNA in the laboratory share general features, such as the use of bacteria and their ...
Date added: June 14, 2015 - Views: 3
Southern blotting, DNA chips, and. FISH . Nucleic Acid Hybridization: DNA Chip. Fig 10.17. ... ANIMATION Dichotomous Keys: Overview. The End. Author: karlm Created Date:
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FISH, Southern transfer (blotting) and DNA microarray analyses involve hybridization. ... Animation. 1-Endo-2-Exo-Cleave . Middle of Chain. The End of the Chain.
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Gel Electrophoresis of DNA ... UV light causes the bands of DNA to fluoresce Animation ... Or DNA can be removed from the gel by Southern Blotting References ...
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See Chapter 9 animation http://bcs ... saliva, bone or any other tissue (often found at a crime scene) Can be done by southern blotting with an appropriate ...
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Cloning Vectors Animation. http://highered.mcgraw-hill.com/sites/0072556781/student_view0/chapter14/animation_quiz_2.html. Storing Cloned Genes in DNA Libraries.
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Chapter 7: Nucleic Acid Amplification Techniques ... agarose gel analysis lacks sensitivity and specificity and Southern blotting is very laborious.
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SOUTHERN BLOTTING The technique was developed by E.M. Southern in 1975. ... Would you watch the animation? A. Yes B. No SUMMARY OF PROCEDURE 1.
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animation. Polymerase Chain Reaction. ... Southern Blotting Technique. The gel is subjected to a chemical that causes the DNA to be denatured.
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In other applications (i.e. Southern blotting), ... The molecular genotypes just described are illustrated in the animation.
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B. Electrophoresis and Blotting. 1. ... Southern blot analyzes DNA . ... http://highered.mcgraw-hill.com/sites/0072556781/student_view0/chapter15/animation_quiz_1.html.
Date added: May 5, 2015 - Views: 8
Title: Snímek 1 Author: Tom McLaren Last modified by: Marie Created Date: 12/1/2002 12:29:48 PM Document presentation format: Předvádění na obrazovce
Date added: October 17, 2011 - Views: 26
Size determined by gel electrophoresis and Southern blotting ... Activities Review tutorial on Western Blot http://tinyurl.com/awaut3 Review VNTR animation http ...
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... isoelectric focusing Two-dimensional polyacrylamide-gel electrophoresis Specific proteins can be detected by Western blotting ... She was a poor Southern ...
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Steps in Southern Blotting Southern Blotting Animation Advantages and Disadvantages of Southern Blotting Advantages The exact location of a disorder does not need ...
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Restriction Enzyme Digestion & Southern Blotting of DNA
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Figure 20.9 Gel electrophoresis Figure 20.9 Gel electrophoresis Figure 20.11 Southern blotting of DNA ... gene Southern blotting animation Northern ...
Date added: September 4, 2011 - Views: 134
Web animation of a cDNA microarray experiment Yeast genome on a chip ... 3’ 3’ – CTTAAG – 5’ (cut) The Southern blotting technique * Title ...
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E.M. Southern Sets the Nomenclature Compass Spinning
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Chapter 20: Biotechnology
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Chapter 20 Biotechnology ... go to Animation and Video ... Gel Electrophoresis and Southern Blotting Gel electrophoresis = method of rapidly analyzing and comparing ...
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After gel electrophoresis Filter paper blots DNA off Radioactive probes added Autoradiography pinpoints sequence Southern Blot animation ... What is Southern blotting ...
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Explain the scientific naming Differentiate between culture, clone, and strain. ... Southern blotting, DNA ... FISH Figs. 10.17 and 10.18 PLAY Animation: ...
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The DNA Toolbox Sequencing of the human genome was completed by 2007 DNA sequencing has depended on advances in technology, starting with making recombinant DNA
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... Manual? 10-13 A Clinical Microbiology Lab Report Form ... What is tested in Western blotting and Southern ... ANIMATION Dichotomous Keys ...
Date added: May 3, 2013 - Views: 74
Chapter 20 Biotechnology ... the DNA of genes Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing ... 2 Animation ...
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... including Southern blotting, ... DNA for recombination Amplify DNA to detectable levels Sequence DNA Diagnose genetic disease Detect pathogens ANIMATION PCR: ...
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* * Figure 15.9: The Southern blotting technique. * Figure 15.9: The Southern blotting technique ... p. 339 1 2 3 4 5 6 7 From blood at crime scene Animation: ...
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Figure 20.10 Restriction fragment analysis by Southern blotting ... How to Insert the Gene of Interest into the R-Plasmid See next 3 figures and animation Lyse ...
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Neutralize gel and place on blotting paper that spans a glass plate ... org/WWW/Marker_pages/CNS/sybII.html Other types of Blotting Techniques: Southern ...
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Chapter 20 Biotechnology ...
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... Scientific Applications Understanding DNA Sequencing organisms' genomes DNA fingerprinting for identification Southern Blotting Southern ... ANIMATION PCR ...
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Chapter 19 Microbial Models: The Genetics of Viruses and Bacteria
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Chapter 3 Methods in Molecular Biology and Genetic Engineering Livet J et al., 2007 Brainbow * 3.9 Blotting Methods Southern blotting involves the transfer of DNA ...
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Chapter 20 Biotechnology
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Chapter 20 Biotechnology
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DNA Technology a Dr. Production ... 2 restriction patterns Interactive Animation, Simplified Blotting Goes from the gel to filter paper to make and autoradiograph ...
Date added: March 4, 2012 - Views: 58
Other Applications of DNA Fingerprinting Parentage test Endangered species or Chinese herbs identification Animation 1: Southern Blotting http://www.dnalc.org ...
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6.3 Advanced Molecular Biological Techniques 1. Polymerase chain reaction (PCR) 2. Restriction fragment length polymorphism (RFLP) 3. DNA sequencing
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ANALYSIS OF DNA BY SOUTHERN BLOTTING The ability to transfer DNA restriction fragments or other DNA molecules that have been separated by gel electrophoresis to ...
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Three examples are: a. Restriction mapping b. Southern blotting c. Northern blotting Restriction Mapping Animation: Restriction Mapping 1.
Date added: June 5, 2012 - Views: 47