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SDS-PAGE and Western blotting

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SDS-PAGE and Western blotting

A Southern blot is a method routinely used in molecular biology for detection of a specific DNA ... SDS-PAGE and Western blotting Author: dhemmerling Last modified ...

http://science.kennesaw.edu/~pachar/Btech3100/Chapter%203-%20SDS-PAGE%20and%20Western%20blotting.ppt

Date added: November 1, 2011 - Views: 273

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Genetics Techniques: RFLP & PCR

Southern Blotting A method ... segment Wash away unbound probe Detect probes using x-ray film autoradiograph RFLP Animations Animation #1 Animation #2 ...

http://mvhs-fuhsd.org/pamela_chow/apbio/lectures/DNA%20Replication%20&%20Genetics/Genetics%20Techniques.ppt

Date added: February 3, 2016 - Views: 1

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Fluorescence in situ Hybridization - University of...

Fluorescence in situ Hybridization Fluorescence in situ Hybridization (FISH) FISH - a process which vividly paints chromosomes or portions of chromosomes with ...

http://www3.nd.edu/~ljordan/butterfly/genetics/fish.ppt

Date added: February 17, 2013 - Views: 221

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Introduction to Biotechnology - Kennesaw State...

Hybridization DNA hybridization Southern blotting ... Primer extension Fig. 3.8 The Polymerase Chain Reaction Go to Animation courtesy of the following websites: ...

http://science.kennesaw.edu/~pachar/Btech3100/Chapter%203%20CONTINUE%20Recombinant%20DNA%20Technology.ppt

Date added: July 1, 2012 - Views: 38

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Gel Electrophoresis of DNA - :: FAPERTA UGM

Gel Electrophoresis of DNA ... UV light causes the bands of DNA to fluoresce Animation ... Or DNA can be removed from the gel by Southern Blotting References ...

http://faperta.ugm.ac.id/download/pak_tar/genetika-molekuler/presentasi/elpho.ppt

Date added: June 16, 2012 - Views: 41

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m.learning.hccs.edu

DNA Cloning and Its Applications: A Preview. Most methods for cloning pieces of DNA in the laboratory share general features, such as the use of bacteria and their ...

http://m.learning.hccs.edu/faculty/muhammed.shakir/biol-1406-power-point/BIOL%201406%20Chapter%2020.pptx

Date added: June 14, 2015 - Views: 16

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Biotechnology and Genetic Engineering-PBIO 450/550

PBIO 427/527: Molecular Genetics Lecture 3 - Review Southern, Northern & Western blotting DNA sequencing Polymerase chain reaction (PCR) DNA microarrays

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20427%20&%20527/PBIO427%20Lecture%203%202009.ppt

Date added: May 4, 2013 - Views: 15

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Biochemical Properties of DNA and The Technology...

Biochemical Properties of DNA and The Technology involve them. ... Animation . 2- Nucleic Acid ... Southern transfer (blotting) and DNA microarray analyses involve ...

http://faculty.ksu.edu.sa/2475/Lectures1/4-Biochemical%20Properties%20of%20DNA%20and%20The%20Technology%20involve%20them.pptx

Date added: February 5, 2012 - Views: 73

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Biotechnology and Genetic Engineering-PBIO 450/550

See Chapter 9 animation http://bcs ... saliva, bone or any other tissue (often found at a crime scene) Can be done by southern blotting with an appropriate ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO4500%20Molecular%20Diagnostics.ppt

Date added: January 21, 2013 - Views: 64

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Forensic DNA Analysis - Southern Oregon University

PCR Animation DNA Fingerprinting Used to identify individuals by their repeat regions (usually STR) regions: Steps involved: ... (Southern blotting) ...

http://webpages.sou.edu/~chapman/CH300S15/Forensic%20DNA%20Analysis%20(student).ppt

Date added: August 4, 2015 - Views: 26

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BIOTECHNOLOGY - anderson1.k12.sc.us

www.dnalc.org/view/15475-The-cycles-of-the-polymerase-chain-reaction-PCR-3D-animation-with-no-audio.html. What DNA Technology Allows. ... Southern Blotting.

http://www.anderson1.k12.sc.us/cms/lib04/SC01000609/Centricity/Domain/1137/campbell%2020%20Biotechnology.pptx

Date added: September 6, 2014 - Views: 14

ppt
PowerPoint Presentation

Southern blotting, DNA chips, and. FISH . Nucleic Acid Hybridization: DNA Chip. Fig 10.17. ... ANIMATION Dichotomous Keys: Overview. The End. Author: karlm Created Date:

https://ashomarconfidential.files.wordpress.com/2011/11/introduction-to-systematic-bacteriology.pptx

Date added: February 9, 2015 - Views: 22

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SOUTHERN BLOTTING - Gadjah Mada University

SOUTHERN BLOTTING The technique was developed by E.M. Southern in 1975. ... Would you watch the animation? A. Yes B. No SUMMARY OF PROCEDURE 1.

http://faperta.ugm.ac.id/download/pak_tar/genetika-molekuler/presentasi/SOUTHERN%20BLOTTING.ppt

Date added: June 25, 2012 - Views: 184

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MICRO. 555 (555 Microbial Molecular Genetics)...

MICRO. 555 (555 Microbial Molecular Genetics) ... 5'-C^TNAG-3') Southern blotting probe ... Sumanas, Inc. animation - DNA electrophoresis & blotting ...

http://faculty.ksu.edu.sa/dr.afafshehata/Lectures/Mic.555/PowerPoint%20%20Mic%20555%20(2).ppt

Date added: October 15, 2011 - Views: 79

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Advanced Molecular Biological Techniques -...

animation. Polymerase Chain Reaction. ... Southern Blotting Technique. The gel is subjected to a chemical that causes the DNA to be denatured.

http://www.stmarycss.ca/parents/teachers/murphy/per3/files/1321973672.pptx

Date added: September 17, 2014 - Views: 1

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PowerPoint Presentation

... Basic method is similar to Southern blotting RNA is ... student_view0/chapter14/animation_quiz_6.html Restriction ... Presentation Author: James ...

http://www.msfta.org/cms/lib6/FL02001163/Centricity/Domain/122/03_Lecture_Presentation.ppt

Date added: February 15, 2015 - Views: 7

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Snímek 1 - old.lf3.cuni.cz

Title: Snímek 1 Author: Tom McLaren Last modified by: Marie Created Date: 12/1/2002 12:29:48 PM Document presentation format: Předvádění na obrazovce

http://old.lf3.cuni.cz/studium/materialy/modul1b/practice_3-rflp.ppt

Date added: October 17, 2011 - Views: 27

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VNTR, STR and RFLP - Austin Community College

Terry Kotrla, MS, MT(ASCP) Objectives Briefly describe VNTR, STR and RFLP Describe the principle, including the target, for: Southern, Western, Northern and ...

http://www.austincc.edu/mlt/mdtech/BlottingMethods.ppt

Date added: September 13, 2011 - Views: 186

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No Slide Title

... isoelectric focusing Two-dimensional polyacrylamide-gel electrophoresis Specific proteins can be detected by Western blotting ... She was a poor Southern ...

http://www.pai.utexas.edu/faculty/isaxena/BIO320/Chapter08%20-%20020911.PPT

Date added: May 27, 2013 - Views: 19

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GENETIC ENGINEERING CHAPTER 20 - spboe.org

B. Electrophoresis and Blotting. 1. ... Southern blot analyzes DNA . ... GENETIC ENGINEERING CHAPTER 20 Last modified by: Karen ...

http://spboe.org/cms/lib3/NJ01001778/Centricity/Domain/718/GENETIC%20ENGINEERING.pptx

Date added: May 5, 2015 - Views: 14

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Fig. 20-1 - Saddleback College

Southern Blot. Short term . ... Animation: Fig. 20-11. TECHNIQUE. Nitrocellulose. membrane (blot) Restriction. fragments. Alkaline. solution. DNA transfer (blotting ...

http://www.saddleback.edu/faculty/thuntley/bio3a/lect/Chapt%2020.pptx

Date added: November 15, 2015 - Views: 2

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Slide 1

Restriction Enzyme Digestion & Southern Blotting of DNA

http://site.iugaza.edu.ps/mlaqqan/files/2013/11/Restriction-enzymes-Southern-blotting-mod.ppt

Date added: December 5, 2013 - Views: 4

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Chapter 20

Chapter 20 Biotechnology You ... gene Destinguish between the Southern blotting ... probe for -globin gene Southern blotting animation Northern blotting animation ...

http://hhh.gavilan.edu/rmorales/documents/20_Lecture_Presentationshortversion.ppt

Date added: September 4, 2011 - Views: 141

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الشريحة 1 - site.iugaza.edu.ps

Restriction Enzyme Digestion & Southern Blotting of DNA 6- Hybridization Steps for hybridization 1. The labeled probe is added to the matrix incubated for several ...

http://site.iugaza.edu.ps/fbalawi/files/2010/02/Exp.-7-Restriction-enzymes-Southern-blotting-mod1.ppt

Date added: September 7, 2011 - Views: 12

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Genetic engineering and the Human Genome Project

Using living microorganisms or cell components to make products Often via genetic engineering and using ... Gel electrophoresis animation What is Southern blotting?

http://skywalker.cochise.edu/ashraft/web/BIO205%20ppt/Lectures%202010/9%20biotechnology.ppt

Date added: May 22, 2013 - Views: 38

ppt
PowerPoint Presentation

Examples of Applications: Southern blotting, DNA chips, and FISH Figs. 10.17 and 10.18 PLAY Animation: Dichotomous Keys (The Microbiology Place) Method ...

http://lpc1.clpccd.cc.ca.us/lpc/zingg/Micro/lecture%20notes/M_T_Ch10_Classification_s.ppt

Date added: June 12, 2012 - Views: 81

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PowerPoint Presentation

... Blotting Southern Blot Transfer Preparation Animation of Transfer Process Crosslink of DNA to Nitrocellulose NC blocking step NC Hybridization Hybridization ...

http://multimedia.mcb.harvard.edu/powerpoint/No-So.ppt

Date added: November 7, 2011 - Views: 32

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PowerPoint Presentation

Web animation of a cDNA microarray experiment Yeast genome on a chip ... 3’ 3’ – CTTAAG – 5’ (cut) The Southern blotting technique * Title ...

http://statwww.epfl.ch/davison/teaching/Microarrays/lec/week02.ppt

Date added: October 15, 2011 - Views: 107

ppt
Chapter 20

Chapter 20 Biotechnology ...

http://www.nscsd.org/webpages/gbroton/files/ch%2020%20biotechnology.ppt

Date added: April 3, 2014 - Views: 7

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DNA Technology Lecture - Green River Community...

Figure 20.10 Restriction fragment analysis by Southern blotting ... How to Insert the Gene of Interest into the R-Plasmid See next 3 figures and animation Lyse ...

http://instruction.greenriver.edu/kmarr/Biology%20211/Lecture%20Notes/Week%2011/1_Ch%2020%20DNA%20Technology_Campbell%206e_F06.ppt

Date added: October 3, 2014 - Views: 34

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Chapter 20

The DNA Toolbox Sequencing of the human genome was completed by 2007 DNA sequencing has depended on advances in technology, starting with making recombinant DNA

http://biologyatmott.weebly.com/uploads/2/6/7/2/2672975/chapter_20_dna_technology.ppt

Date added: May 26, 2014 - Views: 14

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Microbiology

... including Southern blotting, ... DNA for recombination Amplify DNA to detectable levels Sequence DNA Diagnose genetic disease Detect pathogens ANIMATION PCR: ...

http://mrsgonzalezbiologylhs.com/files/73208322.ppt

Date added: August 24, 2014 - Views: 19

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Chapter 7: Nucleic Acid Amplification Techniques

Chapter 7: Nucleic Acid Amplification ... The amplification product then has to be detected by gel electrophoresis and ethidium bromide staining or Southern blotting.

http://www.austincc.edu/mlt/mdfund/mdfund_unit9Chapter7NucleicAcidAmplificationTechniques.ppt

Date added: October 11, 2011 - Views: 167

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chapter_08-09b.ppt - University of Miami

Neutralize gel and place on blotting paper that spans a glass plate ... org/WWW/Marker_pages/CNS/sybII.html Other types of Blotting Techniques: Southern ...

http://www.bio.miami.edu/mccracken/genetics/lectures-2013/chapter_08-09b.ppt

Date added: October 14, 2014 - Views: 4

ppt
PowerPoint Presentation

Examples of Applications: Southern blotting, DNA chips, and FISH Nucleic Acid Hybridization: DNA ... Sample with Flowchart ANIMATION Dichotomous Keys: ...

http://lpc1.clpccd.cc.ca.us/lpc/zingg/Micro/lecture%20notes/M_T_Ch10_Classification_SS10.ppt

Date added: December 5, 2011 - Views: 105

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Microbiology - hccfl.edu

... What is tested in Western blotting and Southern ... a Cladogram Building a Cladogram What is tested in Western blotting and ... ANIMATION Dichotomous Keys ...

http://www.hccfl.edu/media/700873/_start-here_ch10_lecture.ppt

Date added: May 3, 2013 - Views: 94

ppt
PowerPoint Presentation

Other Applications of DNA Fingerprinting Parentage test Endangered species or Chinese herbs identification Animation 1: Southern Blotting http://www.dnalc.org ...

http://www.chuenyuen.edu.hk/~cyc-bio/docs/AL/Electrophoresis%20by%20CUHK.ppt

Date added: March 16, 2012 - Views: 22

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Chapter 20

Chapter 20 Biotechnology ... the DNA of genes Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing ... 2 Animation ...

http://central.rcs.k12.tn.us/Teachers/corbanh/new11_teacher_template/documents/20_Lecture_Presentation_001.ppt

Date added: July 28, 2015 - Views: 4

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Microbiology - Imperial Valley College Faculty...

... Scientific Applications Understanding DNA Sequencing organisms' genomes DNA fingerprinting for identification Southern Blotting Southern ... ANIMATION PCR ...

http://faculty.imperial.edu/samuel.david/bio-220/item/download/56

Date added: November 27, 2013 - Views: 34

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Chapter 20

Chapter 20 Biotechnology

http://learning.hccs.edu/faculty/james.jabbur/biol1406/overhead-material-presented-during-lecture/chapter-20

Date added: April 21, 2016 - Views: 1

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CHAPTER 8 Applications of Recombinant DNA...

Peter J. Russell CHAPTER 8 Applications of Recombinant DNA Technology edited by Yue-Wen Wang Ph. D. Dept. of Agronomy, NTU

http://ntur.lib.ntu.edu.tw/bitstream/246246/2006092815540060/1/00ch8.ppt

Date added: July 10, 2012 - Views: 89

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CHAPTER 8 Recombinant DNA Technology - 草地科學

Southern blotting c. Northern blotting Restriction Mapping Animation: ... CHAPTER 8 Recombinant DNA Technology Author:

http://grassland.agron.ntu.edu.tw/genelab/Class_slide/Peter_Russell/2Ed/Ch8.ppt

Date added: June 5, 2012 - Views: 47

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Microbiology - spaces.imperial.edu

The DNA bands are transferred to a nitrocellulose filter by blotting. ... Figure 9.16 Southern blotting. Forensic Microbiology PCR Primer for ... ANIMATION ...

http://spaces.imperial.edu/sam.david/micro%20chapter%209%20ppt.%2011%20th%20edition.ppt

Date added: March 24, 2014 - Views: 1

ppt
Slide 1

UNIT 4 Techniques used in Molecular Biology

http://utechdmd2015.wikispaces.com/file/view/unit4_Molecular_Biology_techniques_revised_1_.ppt

Date added: March 22, 2012 - Views: 49

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DNA Technology - Dr. Annette M. Parrott

DNA Technology a Dr. Production ... 2 restriction patterns Interactive Animation, Simplified Blotting Goes from the gel to filter paper to make and autoradiograph ...

http://www.africangreyparrott.com/DNATechnology.ppt

Date added: March 4, 2012 - Views: 61

ppt
PowerPoint Presentation

ANALYSIS OF DNA BY SOUTHERN BLOTTING The ability to transfer DNA restriction fragments or other DNA molecules that have been separated by gel electrophoresis to ...

https://lecturesug3.files.wordpress.com/2012/09/lecture-15.ppt

Date added: February 8, 2015 - Views: 9

ppt
PowerPoint Presentation

Chapter 20 Biotechnology

http://www.westex.org/cms/lib6/NJ01001533/Centricity/Domain/101/20_Lecture_Presentation.ppt

Date added: March 25, 2015 - Views: 4

ppt
Slide 1

Southern blotting -- involves 3 key steps 1. Transfer to nitrocellulose 2. “Probe ... Thermocycler Primers Taq-polymerase PCR animation . Title ...

http://www.marietta.edu/~spilatrs/biol131/11-GeneTech.ppt

Date added: May 19, 2013 - Views: 5

ppt
PowerPoint Presentation

Figure 11.4 Southern blotting. ... a predetermined identity is called a nucleic acid probe Southern ... fragments © 2012 Pearson Education, Inc. Animation: ...

http://biology.illinoisstate.edu/wanicho/bsc260/ppt/genetic%20engineering.ppt

Date added: October 17, 2014 - Views: 7