Title: Tissue Culture And Micro Propagation Author: Lawrence F. Iles Last modified by: b715324 Created Date: 9/17/2004 2:36:40 AM Document presentation format
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Plant Tissue Culture Definition: The term plant tissue culture is described in vitro and aseptic cultivation of any plant part on nutrient medium.
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Cell And Tissue Culture Mammalian Cells Advanced Higher Biology Why is it useful? Gene manipulation Culturing mammalian cells for cancer studies Producing new plants ...
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Plant Tissue Culture. A rice plant growing in nutrient rich agar. Tissue Culture. A.K.A. Micropropagation. In vitro Culture.
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Major development’s in cell culture technology Why is cell culture used for? Contd…. Tissue culture Primary culture Continous cell lines Types of cells Culture ...
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Plant Tissue Culture Media What’s really important? Plant Tissue Culture Why does it work? Plant cells – Dedifferentiate Plant cell division- Somatic cells are ...
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Tissue Culture Methodology Historical Perspective Early 20th Century Technique Antibiotics and Laminar Flow Hoods Allow for Tissue Culturing to Grow Exponentially ...
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Introduction. What is Plant Tissue Culture? Plant tissue culture is the aseptic (free from microorganism) culture of any plant part in vitro on an artificially ...
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Tissue Culture. In vitro cultivation of: Organs (organ culture) Tissues (explant culture) Cells (cell culture) Rat aortic explant culture. HUVEC Cells
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Schedule CSES 5233 Spring 09 Jan 13: Introduction/ Tissue culture Jan 15: Agrobacterium Jan 20: Direct DNA transformation Jan 22: Plastid transformation
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Plant Tissues: Overview ... Transitional Meristem Primary growth Protoderm gives rise to epidermis Ground meristem gives rise to ground tissue Procambium gives ...
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Grow mammalian vs bacterial cells. For both need: For bacteria: Tryptone, yeast extract, salt +/- antibiotic, agar. For MES cells: DMEM, calf serum, glutamine, penn/strep
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Bioreactor design Issues for cell cultures Comparison of Chondrogensis in Static and Perfused Bioreactor Culture David Pazzano,† Kathi A. Mercier,†,| John M ...
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Title: Tissue Culture Techniques Lab Author: MAHMOUD Last modified by: Mahmoud Created Date: 9/6/2012 7:46:51 PM Document presentation format: عرض على ...
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Agricultural Biotechnology in Sri Lanka Kumudu Fernando Seed Certification and plant protection Centre, Department of Agriculture Tissue Culture Micropropagation -1 ...
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Plant Tissue Culture Project What is Plant Tissue Culture? Plant tissue culture is a form of asexual propagation of plants under laboratory conditions.
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Culturing (micropropagating) Plant Tissue - the steps. Stage 0 – Selection & preparation of the mother plant. sterilization of the plant tissue takes place
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Genetic variations in plants that have been produced by plant tissue culture and can be ... at low frequency Callus Tissue Organogenesis Regenerated plants ...
Date added: November 21, 2011 - Views: 244
MARKETING STRATEGY FOR SELLING TISSUE CULTURE IN-DOOR POT PLANTS IN GERMANY Dr. Blessed Okole Technology Manager Council For Scientific and Industrial Research
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Tissue culture provided another level of genetic variation: somaclonal variation. Ability to cut and ligate DNA: gene mapping and cloning techniques.
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Plant Tissue Cultures Conditions required for plant tissue cultures An original stock of cells. The use of aseptic techniques when preparing media and cultures.
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Notes on Hosta Tissue Culture John Wessel www.hostatissueculture.com 5 Distinct Phases Of Doing Tissue Culture Preparation of the medium Preparation of Medium ...
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principles of crop production abt-320 (3 credit hours)) lecture 3 lecture-wise course breakup tissue culture major components of tissue culture media
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Basic Principles & Protocol in Plant Tissue Culture Chapter 9 Objective Be familiar with the protocol in plant tissue culture Get know the application of aseptic ...
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Plant Breeding and Applied Genetics ... (Tissue culture, e.g. grapes) (Dr. Sadanand Dhekney) July 8 Molecular Genetics (PCR, Different marker systems) ...
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Plant propagation... Sexual methods - seed propagation Asexual methods rooting cuttings layering; air layering grafting specialized structures tissue culture ...
Date added: October 12, 2011 - Views: 220
Plant Tissue Culture Stage IV gradually increase light intensity and lower RH after rooting occurs allows plants to harden and helps plants form cuticle Cuticle waxy ...
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Applications of plant tissue culture to phytochemistry. Via in vitro mass clonal propagation each single plant cell potentially can generate an entire new plant and ...
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Plant Propagation Jeff Schalau Assoc ... useful when scion wood is limited Stronger union that grafting T Budding Bark must be “slipping” T Budding Tissue Culture ...
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... that supports epithelium--really associated connective tissue Connective tissue support Nutrients from capillaries in ... PowerPoint Presentation Author
Date added: October 9, 2011 - Views: 209
Plant Tissue Culture Media * Auxin and Cytokinin Ratio Gibberellins (GA’s) A family of over 70 related compounds, all forms of Gibberellic acid and named as GA1 ...
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Plant Tissue Culture Media * Abscisic Acid Only one natural compound. Promotes leaf abscission and seed dormancy. Plays a dominant role in closing stomata in response ...
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MICROPROPAGATION This is the bulk production of clonal plants for rapid propagation. It is an important application of tissue culture technology in plant breeding.
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Adenine or adenine-like compounds induce cell division in plant tissue culture. ... or differentiation of vascular tissue, etc.). PLANT GROWTH REGULATORS PLANT ...
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Laboratory Diagnosis of Viral Infection Detection – Isolation ... Virus isolation in tissue culture cell line Viral identification: This is achieved by: ...
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Tissue culture needs extreme aseptic technique! Needs a hood, incubator, equipment NOT being used for things like bacterial growth. Cells need to be under a hood ...
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Plant Tissue Culture 102-1 Prof. H. C Wu History What’s the history? 1838 – Schwann and Schleiden put forward the theory which states that cells are totipotent ...
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Lesson 19 Propagating Plants by Tissue Culture Interest Approach Bring an African violet plant to class. Discuss with students how a commercial grower of violets ...
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Title: Lab #3: Primary Culture Author: MAHMOUD Last modified by: modaa Created Date: 9/20/2012 10:46:22 AM Document presentation format: On-screen Show (4:3)
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Propagating Plants Asexually
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Introduction to Plant Pathology AND environmental impact Bacterial infections The infected head tissue often takes on a tan color Becomes moist and mushy Develops a ...
Date added: September 28, 2012 - Views: 92
Plant and Mammalian Tissue Culture Plant Culture Laboratory - Start Flytrap or Rose Culture Lab Goals Become familiar with Plant Tissue Culture Work aseptically with ...
Date added: August 11, 2013 - Views: 2
Title: Nutritional Requirements of Tissue culture Author: Internet Center Last modified by: Internet Center Created Date: 1/6/2007 9:00:36 AM Document presentation format
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FIGURE 1.1 Growth of Tissue Culture. Number of hits in PubMed for ‘‘cell culture’’ from 1965. The pre-1960 figure is derived from the bibliography of Fischer ...
Date added: October 26, 2013 - Views: 1
Title: Plant Tissue Culture Author: Chiun Last modified by: How-Chiun Wu Created Date: 9/10/2013 1:29:04 AM Document presentation format: On-screen Show (4:3)
Date added: December 27, 2013 - Views: 2
D. A. B. C. Leaf Cutting Grafting Tissue Culture Layering etc. ... Unit A3-3 Author: Leslie Fairchild Last modified by: Leslie Nichole Fairchild Created Date:
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How is Plant Tissue Culture Done? It is grown on a special culture medium which supports its growth and development. The medium can be either semisolid, ...
Date added: April 22, 2014 - Views: 4
Tissue Engineering of the Skin Connor Walsh The Skin The skin is the largest organ in the human body. It consists of about ten percent of our body mass.
Date added: April 25, 2012 - Views: 39
There are six types of in vitro culture types that can create a whole plant: a) Callus culture—culture of differentiated tissue from an explantthat dedifferentiates
Date added: October 12, 2013 - Views: 21